Bai Zhi-Kun, Li Dang-Dang, Guo Chuan-Hui, Yang Zhan-Qing, Cao Hang, Guo Bin, Yue Zhan-Peng
College of Veterinary Medicine, Jilin University, Changchun, 130062, People's Republic of China.
College of Veterinary Medicine, Northeast Agricultural University, Harbin, People's Republic of China.
Cell Tissue Res. 2015 Oct;362(1):231-40. doi: 10.1007/s00441-015-2174-z. Epub 2015 Apr 21.
Runx1 transcription factor is a key developmental regulator. However, little is known about the effects of Runx1 on embryo implantation and decidualization. The aim of this study is to examine the expression and regulation of Runx1 in mouse uterus during the peri-implantation period. There was no evident Runx1 mRNA signal on days 1-4 of pregnancy. On day 5 of pregnancy, Runx1 mRNA was mainly localized in the subluminal stroma surrounding the implanting blastocyst. A similar result was observed in the estrogen-activated implantation uterus. Simultaneously, a high level of Runx1 mRNA expression was detected on days 6-8 of pregnancy and under artificial decidualization. 8-Br-cAMP could induce the expression of Runx1 mRNA in the uterine stromal cells. Moreover, the induction was obviously blocked by PKA inhibitor H89. Inhibition of Runx1 with specific siRNA could decrease the proliferation of stromal cells and expression of decidual markers Prl8a2 and Prl3c1 in the uterine stromal cells. Further study found that inhibition of Runx1 could also suppress the expression of Cox-2, mPGES-1 and Mmp2 genes in uterine stromal cells. Estrogen and progesterone could induce the expression of Runx1 mRNA in ovariectomized mouse uterus and uterine stromal cells. Taken together, these data suggest that Runx1 may play an important role during mouse decidualization.
Runx1转录因子是一种关键的发育调节因子。然而，关于Runx1对胚胎着床和蜕膜化的影响知之甚少。本研究的目的是检测围植入期小鼠子宫中Runx1的表达及调控情况。妊娠第1 - 4天未检测到明显的Runx1 mRNA信号。妊娠第5天，Runx1 mRNA主要定位于围绕着床囊胚的腔下基质中。在雌激素激活的着床子宫中也观察到类似结果。同时，在妊娠第6 - 8天以及人工蜕膜化情况下检测到高水平的Runx1 mRNA表达。8 - Br - cAMP可诱导子宫基质细胞中Runx1 mRNA的表达。此外，PKA抑制剂H89可明显阻断这种诱导作用。用特异性siRNA抑制Runx1可降低基质细胞的增殖以及子宫基质细胞中蜕膜标志物Prl8a2和Prl3c1的表达。进一步研究发现，抑制Runx1还可抑制子宫基质细胞中Cox - 2、mPGES - 1和Mmp2基因的表达。雌激素和孕酮可诱导去卵巢小鼠子宫及子宫基质细胞中Runx1 mRNA的表达。综上所述，这些数据表明Runx1可能在小鼠蜕膜化过程中发挥重要作用。
