Gravina Silvia, Ganapathi Shireen, Vijg Jan
Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Nucleic Acids Res. 2015 Aug 18;43(14):e93. doi: 10.1093/nar/gkv366. Epub 2015 Apr 19.
Stochastic epigenetic changes drive biological processes, such as development, aging and disease. Yet, epigenetic information is typically collected from millions of cells, thereby precluding a more precise understanding of cell-to-cell variability and the pathogenic history of epimutations. Here we present a novel procedure for directly detecting epimutations in DNA methylation patterns using single-cell, locus-specific bisulfite sequencing (SLBS). We show that within gene promoter regions of mouse hepatocytes the epimutation rate is two orders of magnitude higher than the mutation rate.
随机表观遗传变化驱动着诸如发育、衰老和疾病等生物学过程。然而,表观遗传信息通常是从数百万个细胞中收集的,因此无法更精确地了解细胞间的变异性以及表观突变的致病史。在此,我们提出了一种使用单细胞、位点特异性亚硫酸氢盐测序(SLBS)直接检测DNA甲基化模式中表观突变的新方法。我们发现,在小鼠肝细胞的基因启动子区域内,表观突变率比突变率高两个数量级。