National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
101 Institute, Ministry of Civil Affairs, Beijing 100070, PR China.
J Hazard Mater. 2015 Sep 15;295:176-84. doi: 10.1016/j.jhazmat.2015.04.010. Epub 2015 Apr 6.
Biological technology used to treat flue gas is useful to replace conventional treatment, but there is sulfide inhibition. However, no sulfide toxicity effect was observed in haloalkaliphilic bioreactors. The performance of the ethanol-fed bioreactor was better than that of lactate-, glucose-, and formate-fed bioreactor, respectively. To support this result strongly, Illumina MiSeq paired-end sequencing of 16S rRNA gene was applied to investigate the bacterial communities. A total of 389,971 effective sequences were obtained and all of them were assigned to 10,220 operational taxonomic units (OTUs) at a 97% similarity. Bacterial communities in the glucose-fed bioreactor showed the greatest richness and evenness. The highest relative abundance of sulfate-reducing bacteria (SRB) was found in the ethanol-fed bioreactor, which can explain why the performance of the ethanol-fed bioreactor was the best. Different types of SRB, sulfur-oxidizing bacteria, and sulfur-reducing bacteria were detected, indicating that sulfur may be cycled among these microorganisms. Because high-throughput 16S rRNA gene paired-end sequencing has improved resolution of bacterial community analysis, many rare microorganisms were detected, such as Halanaerobium, Halothiobacillus, Desulfonatronum, Syntrophobacter, and Fusibacter. 16S rRNA gene sequencing of these bacteria would provide more functional and phylogenetic information about the bacterial communities.
生物技术用于处理烟气有用以替代传统的处理方法，但存在硫化物抑制。然而，在嗜盐碱性生物反应器中没有观察到硫化物毒性效应。乙醇进料生物反应器的性能优于乳酸盐、葡萄糖和甲酸盐进料生物反应器。为了有力地支持这一结果，应用 Illumina MiSeq 16S rRNA 基因的配对末端测序来研究细菌群落。共获得 389971 条有效序列，所有序列均在 97%相似性水平下被分配到 10220 个操作分类单元 (OTUs)。葡萄糖进料生物反应器中的细菌群落表现出最大的丰富度和均匀度。乙醇进料生物反应器中发现硫酸盐还原菌 (SRB) 的相对丰度最高，这可以解释为什么乙醇进料生物反应器的性能最佳。检测到不同类型的 SRB、硫氧化菌和硫还原菌，表明硫可能在这些微生物之间循环。由于高通量 16S rRNA 基因配对末端测序提高了细菌群落分析的分辨率，检测到了许多稀有微生物，如 Halanaerobium、Halothiobacillus、Desulfonatronum、Syntrophobacter 和 Fusibacter。对这些细菌的 16S rRNA 基因测序将提供关于细菌群落的更多功能和系统发育信息。
