Water Resources Research Institute, Sejong University, 98 Gunja-Dong, Gwangjin-Gu, Seoul 143-747, Republic of Korea.
Department of Civil and Environmental Engineering, Sejong University, 98 Gunja-Dong, Gwangjin-Gu, Seoul 143-747, Republic of Korea.
Sci Total Environ. 2015 Aug 15;524-525:290-9. doi: 10.1016/j.scitotenv.2015.04.017. Epub 2015 Apr 18.
Soil column (SC) experiments were conducted to investigate the feasibility of using silver nanoparticles (AgNPs) as microbial inhibitors; the microbial viability affecting the degradation of pharmaceutically active compounds (PhACs) and the characteristics of organic matter during managed aquifer recharge were specifically evaluated. Natural surface water samples treated with AgNPs (0, 2.5, 5, and 10 mg L(-1)) were continually fed into the soil columns for 2 years. The adverse impact of AgNPs on the cell membrane integrity and microbial enzymatic activity was quantitatively determined using flow cytometry and adenosine triphosphate analysis. The increase in AgNP concentration in the feed water (up to 10 mg L(-1)) resulted in a corresponding deterioration in the performance of the managed aquifer recharge (MAR), with respect to the removal of organic carbon, oxidation of nitrogenous compounds, and PhAC attenuation. The fluorescence excitation-emission matrices of feed water and treated water showed the favorable removal of protein-like substances compared to humic-like substances regardless of the AgNP concentrations; however, the extent of removed fractions decreased noticeably when the microbial viability was lowered via AgNP treatment. The biological oxidation of organic nitrogen was almost completely inhibited when 10 mg L(-1) AgNP was added during soil passage. The attenuation of bezafibrate, ketoprofen, diclofenac, clofibric acid, and gemfibrozil was strongly associated with the significant deterioration in biodegradation as a result of AgNP activity.
进行了土壤柱（SC）实验，以研究使用银纳米粒子（AgNPs）作为微生物抑制剂的可行性；具体评估了影响药物活性化合物（PhACs）降解和含水层管理补给过程中有机物特性的微生物活性。用 AgNPs（0、2.5、5 和 10 mg L（-1））处理的天然地表水样品连续 2 年被注入土壤柱。使用流式细胞术和三磷酸腺苷分析定量测定 AgNPs 对细胞膜完整性和微生物酶活性的不利影响。进水中 AgNP 浓度的增加（高达 10 mg L（-1））导致含水层管理补给（MAR）性能相应恶化，表现在有机碳去除、含氮化合物氧化和 PhAC 衰减方面。无论 AgNP 浓度如何，进水流和处理水流的荧光激发-发射矩阵都显示出有利于去除蛋白样物质而不是腐殖质样物质；但是，当通过 AgNP 处理降低微生物活力时，去除的分数明显减少。当在土壤中添加 10 mg L（-1）AgNP 时，有机氮的生物氧化几乎完全被抑制。苯扎贝特、酮洛芬、双氯芬酸、氯贝酸和吉非贝齐的衰减与 AgNP 活性导致生物降解显著恶化密切相关。
