不同后处理洗涤次数下胆碱离子液体预处理蔗渣的糖化和乙醇发酵。
Saccharification and ethanol fermentation from cholinium ionic liquid-pretreated bagasse with a different number of post-pretreatment washings.
机构信息
Institute of Nature and Environmental Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
Faculty of Natural System, Institute of Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
出版信息
Bioresour Technol. 2015;189:203-209. doi: 10.1016/j.biortech.2015.04.022. Epub 2015 Apr 11.
Choline acetate (ChOAc), a cholinium ionic liquid (IL), was compared with 1-ethyl-3-methylimidazolium acetate (EmimOAc) with regard to biomass pretreatment, inhibition on cellulase and yeast, residuals in pretreated biomass, and saccharification and fermentation of pretreated biomass. Irrespective of ChOAc and EmimOAc, cellulose and hemicellulose saccharification of the IL-pretreated bagasse were over 90% and 60%, respectively. Median effective concentrations (EC50) based on cellulase activity were 32 wt% and 16 wt% for ChOAc and EmimOAc, respectively. The EC50 based on yeast growth were 3.1 wt% and 0.3 wt% for ChOAc and EmimOAc respectively. The residuals in IL-pretreated bagasse were 10% and 23% for ChOAc and EmimOAc, respectively, when washed 2 times after pretreatment. Ethanol yield on a bagasse basis were 60% and 24% for ChOAc and EmimOAc, respectively, in the saccharification and fermentation of IL-pretreated bagasse when washed 2 times. ChOAc-pretreated bagasse could be saccharified and fermented with fewer wash times than EmimOAc-pretreated bagasse.
醋酸胆碱（ChOAc），一种胆碱离子液体（IL），在生物质预处理、纤维素酶和酵母抑制、预处理生物质残留以及预处理生物质的糖化和发酵方面，与 1-乙基-3-甲基咪唑醋酸盐（EmimOAc）进行了比较。无论是 ChOAc 还是 EmimOAc，IL 预处理蔗渣的纤维素和半纤维素糖化率均超过 90%和 60%。基于纤维素酶活性的半数有效浓度（EC50）分别为 32wt%和 16wt%。基于酵母生长的 EC50 分别为 3.1wt%和 0.3wt%。预处理后洗涤 2 次时，ChOAc 和 EmimOAc 预处理蔗渣的残渣分别为 10%和 23%。当 ChOAc 和 EmimOAc 预处理的蔗渣分别洗涤 2 次时，IL 预处理蔗渣的糖化和发酵的乙醇产率分别为 60%和 24%。ChOAc 预处理的蔗渣可以比 EmimOAc 预处理的蔗渣用更少的洗涤次数进行糖化和发酵。
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