Yan Man, An Ya-ting, Li Jian, Wu Zhi-zhen, Wang Tao
Zhongguo Zhong Yao Za Zhi. 2014 Dec;39(24):4856-9.
In this study, SD rats were orally administrated with oteracil potassium (300 mg . kg-1 . d-1 ) to prepare the hyperuricemia model, and divided into normal, model, Allopurinol, LE high dosage, middle dosage and low dose (200, 100, 50 mg . kg-1 . d-1) groups. The rats were orally administrated with test drugs 1 hour later after being orally administrated with Oteracil potassium. After 7 days, serum uric acid, serum creatinine, uric acid and expression of relevant transporters in kidney were tested to study the regulatory effect of leonurus extracts on serum uric acid, renal function and relevant transporters in kidney of rats with hyperuricemia. Compared with the model group, the leonurus extract group could significantly down-regulate serum uric acid and creatinine levels of rats with hyperuricemia, and increase the urine uric acid level. Meanwhile, leonurus extracts could notably down-regulate the mRNA expressions of urate transporter 1 (URAT1) and glucose transporter 9 (GLUT9), up-regulate the mRNA expressions of organic cation transportanter (OCT) and Carnitine transporter (OCTN) and promote the excretion of uric acid of kidney.
本研究中，给SD大鼠灌胃奥替拉西钾（300 mg·kg-1·d-1）制备高尿酸血症模型，并分为正常组、模型组、别嘌醇组、益母草提取物高剂量组、中剂量组和低剂量组（200、100、50 mg·kg-1·d-1）。大鼠灌胃奥替拉西钾1小时后灌胃给予受试药物。7天后，检测血清尿酸、血清肌酐、尿尿酸及肾脏相关转运体的表达，以研究益母草提取物对高尿酸血症大鼠血清尿酸、肾功能及肾脏相关转运体的调节作用。与模型组相比，益母草提取物组可显著下调高尿酸血症大鼠的血清尿酸和肌酐水平，并增加尿尿酸水平。同时，益母草提取物可显著下调尿酸转运体1（URAT1）和葡萄糖转运体9（GLUT9）的mRNA表达，上调有机阳离子转运体（OCT）和肉碱转运体（OCTN）的mRNA表达，促进肾脏尿酸排泄。
