Collet C, Joseph R, Nicholas K
CSIRO Division of Wildlife and Ecology, Lyneham, A.C.T., Australia.
Biochem Biophys Res Commun. 1989 Nov 15;164(3):1380-3. doi: 10.1016/0006-291x(89)91822-6.
The tammar wallaby (Macropus eugenii) produces two different types of milk; "early" milk for the first 180 days and "late" milk for the remaining 150 days of lactation. We report the cloning of the cDNA of a novel whey protein, Late-Lactation Protein (LLP), which is produced during the late phase of lactation. The cDNA was cloned using an oligonucleotide probe deduced from the published partial protein sequence. The cDNA encodes an open-reading frame of 176 amino acids of which the first 18 residues represent a signal peptide. Northern blot analysis revealed a 1kb transcript in late phase lactation but not in the early phase.
帚尾袋貂（Macropus eugenii）能分泌两种不同类型的乳汁：哺乳期前180天分泌的“早期”乳汁和剩余150天分泌的“晚期”乳汁。我们报告了一种新型乳清蛋白——晚期泌乳蛋白（LLP）的cDNA克隆，该蛋白在泌乳后期产生。利用从已发表的部分蛋白质序列推导的寡核苷酸探针克隆了该cDNA。该cDNA编码一个由176个氨基酸组成的开放阅读框，其中前18个残基代表信号肽。Northern印迹分析显示，晚期泌乳期有一个1kb的转录本，而早期没有。
