Maillot-Vernier P, Schaller H, Benveniste P, Belliard G
UA 1182, Laboratoire de Biologie et Biochimie du Développement des Plantes, Institut de Botanique, Strasbourg Cedex, France.
Biochem Biophys Res Commun. 1989 Nov 30;165(1):125-30. doi: 10.1016/0006-291x(89)91043-7.
We report here, for the first time, the biochemical characterization of a plant mutant impaired in sterol biosynthesis. A fertile plant was regenerated from a tobacco callus resistant to LAB170250F, a potent inhibitor of the cytochrome-P450-obtusifoliol-14-demthylase. The resistant callus and the leaves from the regenerated plant are characterized by profound qualitative and quantitative changes in their sterol content. Self-fertilization of this plant yielded seeds with the same biochemical features, indicating that the new phenotype is of mutational origin.
我们首次在此报告一种在甾醇生物合成中受损的植物突变体的生化特征。从对细胞色素-P450-钝叶醇-14-脱甲基酶的强效抑制剂LAB170250F具有抗性的烟草愈伤组织中再生出了一株可育植株。抗性愈伤组织和再生植株的叶片在甾醇含量上表现出深刻的定性和定量变化。该植株自交产生的种子具有相同的生化特征，表明这种新表型源自突变。
