Foti Debra, Zhang Lei, Biswas Preetha, Mozola Mark, Rice Jennifer
Neogen Corp., 620 Lesher Pl, Lansing, MI 48912, USA.
J AOAC Int. 2014 Sep-Oct;97(5):1374-83. doi: 10.5740/jaoacint.14-012.
A matrix extension study was conducted to validate the ANSR(®) Salmonella method for use with pasteurized egg products. Four diverse egg product types were tested by the ANSR method and the U.S. Department of Agriculture's Microbiology Laboratory Guidebook reference culture procedure. There were no significant differences in the number of positive test portions by the two methods for any of the products examined. Independent laboratory testing of pasteurized liquid egg also found no significant difference in performance between the ANSR and reference culture procedures. There were no false-positive results obtained in either internal or independent laboratory testing. Inclusivity testing using a new enrichment medium specifically designed for use with pasteurized egg products produced 112 positive results out of 113 Salmonella spp. strains tested, with only a single strain of S. Weslaco testing negative by the ANSR assay. Exclusivity testing of 38 non-salmonellae produced all negative ANSR results. It is concluded that ANSR Salmonella is a reliable, sensitive, and specific method for detection of Salmonella spp. in pasteurized egg products.
进行了一项基质扩展研究，以验证用于巴氏杀菌蛋制品的ANSR（®）沙门氏菌检测方法。通过ANSR方法和美国农业部微生物学实验室指南参考培养程序对四种不同类型的蛋制品进行了检测。对于所检测的任何产品，两种方法的阳性检测部分数量均无显著差异。对巴氏杀菌液态蛋进行的独立实验室检测也发现，ANSR方法与参考培养程序在性能上没有显著差异。内部或独立实验室检测均未获得假阳性结果。使用专门为巴氏杀菌蛋制品设计的新型增菌培养基进行的包容性检测，在113株测试的沙门氏菌菌株中产生了112个阳性结果，只有一株韦斯拉科沙门氏菌通过ANSR检测呈阴性。对38株非沙门氏菌进行的排他性检测，ANSR结果均为阴性。结论是，ANSR沙门氏菌检测方法是检测巴氏杀菌蛋制品中沙门氏菌的可靠、灵敏且特异的方法。
