Zhao Haixiang, Ding Mingyu, Gao Yunbao, Deng Wei
Hebei North University, College of Science, Zhangjiakou 075000, People's Republic of China.
J AOAC Int. 2014 Sep-Oct;97(5):1481-8. doi: 10.5740/jaoacint.13-133.
Twelve sulfonamide residues, namely, sulfadiazine, sulfamerazine, sulfamethazine, sulfamonomethoxine, sulfadoxine, sulfamethoxazole, sulfaquinoxaline, sulfadimethoxine, sulfanilamide, sulfathiazole, sulfapyridine, and sulfamethizole were determined in pork tissue, chicken, and eggs by ultra-performance LC/MS/MS after SPE cleanup using multiwalled carbon nanotubes, which permitted extracted samples from animal tissue and eggs to be cleaned up under either normal phase (NP) or reversed phase (RP) conditions or both. In this method, the NP cleanup provided recoveries for nine of the 12 sulfonamides ranging from 71 to 90% and <60% for the remaining three with RSDs lower than 10%. For the RP cleanup method, the recoveries for nine of the 12 sulfonamides ranged from 70 to 94% and <60% for the remaining three with RSDs <11.1%. The two cleanup methods can complement each other to complete the simultaneous analysis of the 12 sulfonamides.
采用多壁碳纳米管进行固相萃取净化后，通过超高效液相色谱/串联质谱法测定了猪肉组织、鸡肉和鸡蛋中的12种磺胺类残留，即磺胺嘧啶、磺胺甲基嘧啶、磺胺二甲嘧啶、磺胺间甲氧嘧啶、磺胺多辛、磺胺甲恶唑、磺胺喹恶啉、磺胺二甲氧嘧啶、磺胺、磺胺噻唑、磺胺吡啶和磺胺甲噻二唑。该方法允许在正相（NP）或反相（RP）条件下或两者同时对动物组织和鸡蛋中的提取物进行净化。在此方法中，正相净化法对12种磺胺类中的9种回收率为71%至90%，其余3种回收率低于60%，相对标准偏差低于10%。对于反相净化法，12种磺胺类中的9种回收率为70%至94%，其余3种回收率低于60%，相对标准偏差小于11.1%。两种净化方法可相互补充，以完成12种磺胺类的同时分析。
