Crawford R J, Hammond V E, Roche P J, Johnston P D, Tregear G W
Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Victoria, Australia.
J Mol Endocrinol. 1989 Nov;3(3):169-74. doi: 10.1677/jme.0.0030169.
The gene encoding rhesus monkey relaxin has been investigated. A cDNA library was prepared using corpus luteal RNA from a pregnant rhesus monkey, cDNA clones encoding relaxin were isolated and the nucleotide sequence was determined. The amino acid sequence of rhesus monkey preprorelaxin, predicted from the cDNA, demonstrates that the sequence has not been strongly conserved when compared with that of man, although features characteristic of the relaxin molecule have been maintained. This structural information will allow production of rhesus monkey relaxin, leading to studies investigating the bioactivity of relaxin in a homologous primate system. Southern blot analysis indicated that there is only one relaxin gene in the rhesus monkey and baboon genomes. In this respect these primate genomes are different from the human genome which contains two relaxin genes.
对恒河猴松弛素的编码基因进行了研究。利用怀孕恒河猴的黄体RNA制备了一个cDNA文库，分离出编码松弛素的cDNA克隆并测定了核苷酸序列。从cDNA预测的恒河猴前松弛素原的氨基酸序列表明，与人类相比，该序列的保守性不强，尽管松弛素分子的特征得以保留。这一结构信息将有助于生产恒河猴松弛素，从而开展在同源灵长类系统中研究松弛素生物活性的相关研究。Southern印迹分析表明，恒河猴和狒狒基因组中仅存在一个松弛素基因。在这方面，这些灵长类动物的基因组与含有两个松弛素基因的人类基因组不同。
