Hatipoglu Manolya Kukut, Keleştemur Seda, Altunbek Mine, Culha Mustafa
Nanotechnology. 2015 May 15;26(19):195103. doi: 10.1088/0957-4484/26/19/195103.
Silver nanoparticles (AgNPs) are increasingly used in a variety of applications because of their potential antimicrobial activity and their plasmonic and conductivity properties. In this study, we investigated the source of cytotoxicity, genotoxicity, and reactive oxygen species (ROS) production on human dermal fibroblast and human lung cancer (A549) cell lines upon exposure to AgNP colloidal suspensions prepared with the simplest and most commonly used Lee–Meisel method with a variety of reaction times and the concentrations of the reducing agent. The AgNPs synthesized with shorter reaction times were more cytotoxic and genotoxic due to the presence of a few nanometer-sized AgNP seeds. The suspensions prepared with an increased citrate concentration were not cytotoxic, but they induced more ROS generation on A549 cells due to the high citrate concentration. The genotoxicity of the suspension decreased significantly at the higher citrate concentrations. The analysis of both transmission electron microscopy images from the dried droplet areas of the colloidal suspensions and toxicity data indicated that the AgNP seeds were the major source of toxicity. The completion of the nucleation step and the formation of larger AgNPs effectively decreased the toxicity.
由于银纳米颗粒（AgNPs）具有潜在的抗菌活性以及等离子体和导电特性，它们在各种应用中的使用越来越广泛。在本研究中，我们研究了在使用最简单且最常用的Lee-Meisel方法、不同反应时间和还原剂浓度制备的AgNP胶体悬浮液作用下，人皮肤成纤维细胞和人肺癌（A549）细胞系产生细胞毒性、遗传毒性和活性氧（ROS）的来源。由于存在几纳米大小的AgNP种子，反应时间较短时合成的AgNPs具有更高的细胞毒性和遗传毒性。用增加的柠檬酸盐浓度制备的悬浮液没有细胞毒性，但由于柠檬酸盐浓度高，它们在A549细胞上诱导产生更多的ROS。在较高的柠檬酸盐浓度下，悬浮液的遗传毒性显著降低。对胶体悬浮液干燥液滴区域的透射电子显微镜图像和毒性数据的分析表明，AgNP种子是毒性的主要来源。成核步骤的完成和更大尺寸AgNPs的形成有效地降低了毒性。
