伊朗德黑兰大学医院医护人员中莫匹罗星耐药性及耐甲氧西林金黄色葡萄球菌鼻腔携带情况的检测

The Detection of Mupirocin Resistance and Nasal Carriage of Methicillin Resistant Staphylococcus aureus among Healthcare Workers at University Hospitals of Tehran, Iran.

作者信息

Ohadian Moghadam Solmaz, Pourmand Mohammad Reza, Davoodabadi Abolfazl

机构信息

Dept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Dept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran ; Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran.

出版信息

Iran J Public Health. 2015 Mar;44(3):361-8.

PMID:25905079

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4402414/

Abstract

BACKGROUND

Nasal mupirocin is found to eradicate effectively methicillin-resistant Staphylococcus aureus (MRSA) from colonized patients, but there are concerns about resistant strains. The aim of this study was to detect the mupirocin resistance and nasal carriage of methicillin resistant S. aureus among healthcare workers (HCWS) at the university hospitals of Tehran.

METHODS

Totally 270 nasal swabs were collected and S. aureus were identified by confirmatory tests in 2013. Determination of oxacillin and mupirocin resistance was performed by disk diffusion method and the mupirocin MIC assessed using E-test. The ileS-2 (mupA) and mecA genes were detected in DNA extracts by multiplex PCR.

RESULTS

The prevalence of S. aureus nasal carriage among HCWs was 14.44%. E-test and disk diffusion methods showed 5 and 4 mupirocin resistant isolates, respectively. Statistically significant difference was observed between sex (P=0.035), hospitals (P=0.0001) and occupation (P=0.009) with nasal carriage of S. aureus. A significant difference was found between sex (P=0.041) and occupation (0.034) with regard to MRSA carriage. All MRSA isolates were susceptible to linezolid, fusidic acid and vancomycin.

CONCLUSION

Since the HCWs play an important role as a reservoir for resistant isolates in the hospital setting, regularly screening should be performed for identification of nasal carriers.

摘要

背景

已发现鼻腔用莫匹罗星可有效清除定植患者体内的耐甲氧西林金黄色葡萄球菌（MRSA），但人们担心会出现耐药菌株。本研究的目的是检测德黑兰大学医院医护人员中莫匹罗星耐药情况及耐甲氧西林金黄色葡萄球菌的鼻腔携带情况。

方法

2013年共收集270份鼻拭子，并通过确证试验鉴定金黄色葡萄球菌。采用纸片扩散法测定苯唑西林和莫匹罗星耐药性，并用E-test法评估莫匹罗星的最低抑菌浓度（MIC）。通过多重聚合酶链反应（PCR）在DNA提取物中检测ileS-2（mupA）和mecA基因。

结果

医护人员中金黄色葡萄球菌鼻腔携带率为14.44%。E-test法和纸片扩散法分别显示有5株和4株莫匹罗星耐药菌株。在金黄色葡萄球菌鼻腔携带情况方面，性别（P=0.035）、医院（P=0.0001）和职业（P=0.009）之间存在统计学显著差异。在MRSA携带情况方面，性别（P=0.041）和职业（P=0.034）之间存在显著差异。所有MRSA分离株对利奈唑胺、夫西地酸和万古霉素敏感。