Sedaghat Hossein, Esfahani Bahram Nasr, Halaji Mehrdad, Jazi Azhar Sallari, Mobasherizadeh Sina, Havaei Seyed Rohollah, Emaneini Mohammad, Havaei Seyed Asghar
Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Iran J Microbiol. 2018 Apr;10(2):82-89.
is an important pathogen that can be colonized in the nose and increase the risk of spreading infections in hospitals. The present study aimed at determining the phenotypic and genotypic characterization of strains isolated from patients and healthcare workers (HCWs) from a teaching hospital in Isfahan, Iran.
This cross-sectional study was performed on 262 nasal swabs and 23 clinical isolates that were collected from a teaching hospital during February and April 2016. Staphylococcal cassette chromosome (SCC) and multilocus sequence typing (MLST) were performed for selected isolates.
Overall, 23% and 18% of healthcare workers and patients were carriers, respectively. Methicillin-resistant (MRSA) rate was 13%, 33% and 52% in nasal HCWs, nasal patients, and clinical samples, respectively. The molecular typing of MRSA isolates revealed that the most common SCC type is SCC type III (88%). The highest rate of resistance was observed against tetracycline and erythromycin, with 48.7%. The most frequently detected toxin genes among isolates were (99%) and (44%), moreover, genes were detected in (40%) of MRSA isolates. The results of MLST showed 7 different sequence types (STs): ST859 (2/9), ST6 (2/9), ST639 (1/9), ST343 (1/9), ST239 (1/9), ST291 (1/9) and ST25 (1/9).
Our results revealed that ST clones associated with healthcare-associated MRSA (HA-MRSA) are actively circulating among nasal carriage in our healthcare setting, and thus, effective infection control policies are needed to reduce nasal carriage in healthcare settings.
是一种重要的病原体，可在鼻腔定植并增加医院感染传播的风险。本研究旨在确定从伊朗伊斯法罕一家教学医院的患者和医护人员（HCWs）中分离出的菌株的表型和基因型特征。
本横断面研究对2016年2月至4月从一家教学医院收集的262份鼻拭子和23份临床分离株进行。对选定的分离株进行葡萄球菌盒式染色体（SCC）和多位点序列分型（MLST）。
总体而言，医护人员和患者的携带者比例分别为23%和18%。耐甲氧西林（MRSA）率在鼻腔医护人员、鼻腔患者和临床样本中分别为13%、33%和52%。MRSA分离株的分子分型显示，最常见的SCC类型是III型SCC（88%）。观察到对四环素和红霉素的耐药率最高，为48.7%。在分离株中最常检测到的毒素基因是（99%）和（44%），此外，在40%的MRSA分离株中检测到基因。MLST结果显示有7种不同的序列类型（STs）：ST859（2/9）、ST6（2/9）、ST639（1/9）、ST343（1/9）、ST239（1/9）、ST291（1/9）和ST25（1/9）。
我们的结果表明，与医疗保健相关的MRSA（HA-MRSA）相关的ST克隆在我们的医疗环境中的鼻腔携带者中活跃传播，因此，需要有效的感染控制政策来减少医疗环境中的鼻腔定植。
