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嗜热栖热菌的七聚体SmAP1和SmAP2蛋白与常见和不同的RNA靶标结合。

The Heptameric SmAP1 and SmAP2 Proteins of the Crenarchaeon Sulfolobus Solfataricus Bind to Common and Distinct RNA Targets.

作者信息

Märtens Birgit, Bezerra Gustavo Arruda, Kreuter Mathias Josef, Grishkovskaya Irina, Manica Andrea, Arkhipova Valentina, Djinovic-Carugo Kristina, Bläsi Udo

机构信息

Department of Microbiology, Immunobiology & Genetics, Max F. Perutz Laboratories, University of Vienna, Vienna Biocenter, Dr. Bohrgasse 9, 1030 Vienna, Austria.

Department of Structural & Computational Biology, Max F. Perutz Laboratories, University of Vienna, Vienna Biocenter, Vienna Biocenter Campus 5, 1030 Vienna, Austria.

出版信息

Life (Basel). 2015 Apr 21;5(2):1264-81. doi: 10.3390/life5021264.

DOI:10.3390/life5021264
PMID:25905548
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4500138/
Abstract

Sm and Sm-like proteins represent an evolutionarily conserved family with key roles in RNA metabolism. Sm-based regulation is diverse and can range in scope from eukaryotic mRNA splicing to bacterial quorum sensing, with at least one step in these processes being mediated by an RNA-associated molecular assembly built on Sm proteins. Despite the availability of several 3D-structures of Sm-like archaeal proteins (SmAPs), their function has remained elusive. The aim of this study was to shed light on the function of SmAP1 and SmAP2 of the crenarchaeon Sulfolobus solfataricus (Sso). Using co-purification followed by RNASeq different classes of non-coding RNAs and mRNAs were identified that co-purified either with both paralogues or solely with Sso-SmAP1 or Sso-SmAP2. The large number of associated intron-containing tRNAs and tRNA/rRNA modifying RNAs may suggest a role of the two Sso-SmAPs in tRNA/rRNA processing. Moreover, the 3D structure of Sso-SmAP2 was elucidated. Like Sso-SmAP1, Sso-SmAP2 forms homoheptamers. The binding of both proteins to distinct RNA substrates is discussed in terms of surface conservation, structural differences in the RNA binding sites and differences in the electrostatic surface potential of the two Sso-SmAP proteins. Taken together, this study may hint to common and different functions of both Sso-SmAPs in Sso RNA metabolism.

摘要

Sm 蛋白和 Sm 样蛋白代表了一个在 RNA 代谢中起关键作用的进化保守家族。基于 Sm 的调控具有多样性，范围从真核生物 mRNA 剪接到细菌群体感应，这些过程中至少有一个步骤是由基于 Sm 蛋白构建的 RNA 相关分子组装介导的。尽管已有几种古菌 Sm 样蛋白（SmAPs）的三维结构，但它们的功能仍然难以捉摸。本研究的目的是阐明嗜热栖热菌（Sulfolobus solfataricus，Sso）的 SmAP1 和 SmAP2 的功能。通过共纯化后进行 RNA 测序，鉴定出了不同类别的非编码 RNA 和 mRNA，它们要么与这两个旁系同源物共同纯化，要么仅与 Sso-SmAP1 或 Sso-SmAP2 共同纯化。大量相关的含内含子 tRNA 和 tRNA/rRNA 修饰 RNA 可能表明这两个 Sso-SmAP 在 tRNA/rRNA 加工中起作用。此外，还阐明了 Sso-SmAP2 的三维结构。与 Sso-SmAP1 一样，Sso-SmAP2 形成同七聚体。从表面保守性、RNA 结合位点的结构差异以及这两种 Sso-SmAP 蛋白的静电表面电位差异等方面讨论了这两种蛋白与不同 RNA 底物的结合情况。综上所述，本研究可能暗示了 Sso 中这两种 Sso-SmAP 在 RNA 代谢中的共同和不同功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d20/4500138/ad524abce18b/life-05-01264-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d20/4500138/3a0863e34265/life-05-01264-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d20/4500138/084a687870db/life-05-01264-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d20/4500138/50d7bd91e0cf/life-05-01264-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d20/4500138/4d455dfef92c/life-05-01264-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d20/4500138/a1ee4b2a3d85/life-05-01264-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d20/4500138/12dfaff327e8/life-05-01264-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d20/4500138/ad524abce18b/life-05-01264-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d20/4500138/3a0863e34265/life-05-01264-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d20/4500138/084a687870db/life-05-01264-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d20/4500138/50d7bd91e0cf/life-05-01264-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d20/4500138/4d455dfef92c/life-05-01264-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d20/4500138/a1ee4b2a3d85/life-05-01264-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d20/4500138/12dfaff327e8/life-05-01264-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d20/4500138/ad524abce18b/life-05-01264-g007.jpg

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