Han Qianqian, Wang Kewei, Xu Lijun, Yan Xiang, Zhang Kunchi, Chen Xing, Wang Qinglin, Zhang Lan, Pei Renjun
Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Collaborative Innovation Center of Suzhou Nano Science and Technology, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
Analyst. 2015 Jun 21;140(12):4143-7. doi: 10.1039/c5an00008d. Epub 2015 Apr 23.
A novel photoelectrochemical (PEC) biosensor was fabricated based on N-doped TiO2 for the detection of Hg(2+) through the quenching of photogenerated electrons. The N-doped TiO2 was synthesized by a sol-gel method with urea and tetrabutyl titanate as the N and Ti sources. Compared with the undoped TiO2, the N-doped TiO2 showed an enhanced photocurrent response under visible light (λ > 420 nm). The sensing surface was functionalized with 5'-amino-modified T-rich oligonucleotides. The photoelectrochemical biosensor bound Hg(2+) on the surface by a highly specific T-Hg(2+)-T recognition. Hg(2+) on the surface of the N-doped TiO2 film withdrew the photogenerated electrons and decreased the recorded current signal. The dynamic linear range for Hg(2+) has been determined to be as low as 2-6 μM.
基于氮掺杂二氧化钛制备了一种新型光电化学（PEC）生物传感器，用于通过光生电子猝灭检测汞离子（Hg(2+)）。以尿素和钛酸四丁酯作为氮源和钛源，采用溶胶-凝胶法合成了氮掺杂二氧化钛。与未掺杂的二氧化钛相比，氮掺杂二氧化钛在可见光（λ > 420 nm）下表现出增强的光电流响应。传感表面用5'-氨基修饰的富含胸腺嘧啶的寡核苷酸进行功能化。该光电化学生物传感器通过高度特异性的T-Hg(2+)-T识别在表面结合Hg(2+)。氮掺杂二氧化钛薄膜表面的Hg(2+)吸引光生电子并降低记录的电流信号。Hg(2+)的动态线性范围已确定低至2 - 6 μM。
