胰管支架的微生物定植：一项前瞻性分析。

Microbial colonization of pancreatic duct stents: a prospective analysis.

作者信息

Schneider Jochen, Schenk Philipp, Obermeier Andreas, Fremd Julia, Feihl Susanne, Forkl Stefanie, Wantia Nina, Römmler Franziska, Neu Bruno, Bajbouj Monther, von Delius Stefan, Schmid Roland M, Algül Hana, Weber Andreas

机构信息

From the *II. Medizinische Klinik und Poliklinik, Klinikum rechts der Isar, Technische Universität München; †Institut für Mikrobiologie, Immunologie und Hygiene, Technische Universität München; and ‡Klinik für Orthopädie und Sportorthopädie, Klinikum rechts der Isar, Technische Universität München, München, Germany.

出版信息

Pancreas. 2015 Jul;44(5):786-90. doi: 10.1097/MPA.0000000000000332.

DOI:10.1097/MPA.0000000000000332

PMID:25906452

Abstract

OBJECTIVE

The aim of the study was to analyze the microbial colonization rate as well as the spectrum and number of microorganisms in relation to the indwelling time of pancreatic stents.

METHODS

Forty pancreatic stents were prepared according to a standardized protocol and subsequently sonicated to optimize bacterial release from the biofilm on the stents.

RESULTS

Two hundred forty-six microorganisms were identified. Thirty-nine of 40 stents were colonized with microorganisms. Aerobic gram-positive microorganisms (106/246 [43%]) accounted for the greatest proportion. The predominant microorganisms were Streptococcus species (46/246 [19%]), which were isolated from 27 (68%) of 40 stents. Stents with a short indwelling time (3-13 days) were mainly colonized with aerobic gram-positive bacteria (82%) and Candida species (63%). In contrast, anaerobes (P < 0.01, 69% vs 18%) and aerobic gram-negative microorganisms (P < 0.01, 93% vs 45%) such as Enterobacteriaceae (P < 0.01, 86% vs 27%) were significantly more present on stents with a long indwelling time (29-93 days), compared with stents with a short indwelling time.

CONCLUSIONS

Microbial analysis of pancreatic duct stents revealed a very high colonization rate. Furthermore, the spectrum and number of microorganisms altered with the indwelling time of the stent. However, clinical relevance of our findings remains unclear.

摘要

目的

本研究旨在分析胰腺支架留置时间与微生物定植率、微生物谱及数量之间的关系。

方法

按照标准化方案制备40个胰腺支架，随后进行超声处理以优化支架生物膜上细菌的释放。

结果

共鉴定出246株微生物。40个支架中有39个被微生物定植。需氧革兰氏阳性微生物（106/246 [43%]）占比最大。主要微生物为链球菌属（46/246 [19%]），从40个支架中的27个（68%）分离得到。留置时间短（3 - 13天）的支架主要被需氧革兰氏阳性菌（82%）和念珠菌属（63%）定植。相比之下，留置时间长（29 - 93天）的支架上厌氧菌（P < 0.01，69%对18%）和需氧革兰氏阴性微生物（P < 0.01，93%对45%）如肠杆菌科（P < 0.01，86%对27%）明显更多。