[麦粒灸对双转基因AD小鼠前额叶皮质和海马中β淀粉样蛋白（1-42）表达的影响]

[Effects of grain-sized moxibustion on expression of Abeta(1-42) in prefrontal cortex and hippocampus in double-transgenic AD mice].

作者信息

Bao Yehua, Zhang Yongsheong, Chu Jiamei, Zeng Youhua, Li Liping, Gao Ling'ai, Yu Jing

出版信息

Zhongguo Zhen Jiu. 2015 Jan;35(1):59-65.

Abstract

OBJECTIVE

To explore the therapeutic effect of grain-sized moxibustion at "Xinshu" (BL 15) and "Shenshu" (BL 23) on early-stage Alzheimer's disease (AD) in transgenosis AD mice.

METHODS

The genotyping of amyloid precursor protein/presenilin 1(APP/PS1I) double-transgenic AD mice were detected by PCR method. Seventeen 1.5-month female transgenic (Tg 6799) mice were randomly divided into a model group (9 cases) and a treatment group (8 cases). Nine female C57BL/6J wild-type mice with identical age and background were selected into a normal group. The treatment group was treated with grain-sized moxibustion at bilateral "Xinshu" (BI. 15) and "Shenshu" (BL 23), once a day, ten treatments were considered as one course, and total 9 courses were given. The model group and normal group were treated with stimulus such as grabbing, immobilization and non-ignited moxa cone. Morris water maze (escape latency, crossing times and dwell time in the target quadrant) was applied to evaluate the learning and memory ability. Hematoxylin-Eosin (HE) staining was used to observe morphology changes in the brains of AD mice. beta-amyloid protein 1-42 (Abeta(1-42)) in the area of prefrontal cortex and hippocampus was detected by immunohistochemical method.

RESULTS

After the treatment of grain-sized moxibustion, learning and memory ability in the treatment group was increased; compared with the model group, the escape latency was shorten, crossing times was increased, and dwell time in the target quadrant was prolonged (all P<0. 05). The crossing times and dwell time in the target quadrant in the treatment group were not significantly different from those in the normal group (both P>0.05). Compared with the normal group, the positive area and integral optical density of Abeta(1-42) in prefrontal cortex and hippocampus in the model group were increased (all P<0.01). Compared with the model group, the positive area and integral optical density of Abeta(1-42) in prefrontal cortex and hippocampus in the treatment group were reduced (P<0.01, P<0.05).

CONCLUSION

The grain-sized moxibustion at "Xinshu" (BL 15) and "Shenshu" (BL 23) could significantly improve the learning and memory ability in APP/PS1 double- transgenic AD mice, and inhibit the over expression and accumulation of Abeta(1-42).

摘要

目的

探讨麦粒灸心俞（BL 15）、肾俞（BL 23）对转基因AD小鼠早期阿尔茨海默病（AD）的治疗作用。

方法

采用聚合酶链反应（PCR）法对淀粉样前体蛋白/早老素1（APP/PS1I）双转基因AD小鼠进行基因分型。将17只1.5月龄雌性转基因（Tg 6799）小鼠随机分为模型组（9只）和治疗组（8只）。选取9只年龄和背景相同的雌性C57BL/6J野生型小鼠作为正常组。治疗组采用麦粒灸双侧心俞（BL 15）、肾俞（BL 23），每日1次；10次治疗为1个疗程，共进行9个疗程。模型组和正常组采用抓取、固定、非点燃艾炷等刺激方式。采用Morris水迷宫（逃避潜伏期、穿越次数和目标象限停留时间）评估学习记忆能力。采用苏木精-伊红（HE）染色观察AD小鼠脑形态学变化。采用免疫组化法检测前额叶皮质和海马区β淀粉样蛋白1-42（Aβ（1-42））。

结果

麦粒灸治疗后，治疗组学习记忆能力增强；与模型组相比，逃避潜伏期缩短，穿越次数增加，目标象限停留时间延长（均P<0.05）。治疗组目标象限穿越次数和停留时间与正常组比较差异无统计学意义（均P>0.05）。与正常组比较，模型组前额叶皮质和海马区Aβ（1-42）阳性面积和积分光密度增加（均P<0.01）。与模型组比较，治疗组前额叶皮质和海马区Aβ（1-42）阳性面积和积分光密度降低（P<0.01，P<0.05）。