Gao Ying, Liu Xiaoyun, Qi Wenjing, Gao Wenyue, Li Yunhui, Xu Guobao
School of Chemistry and Environmental Engineering, Changchun University of Science and Technology, Changchun 130022, China.
Analyst. 2015 Jun 21;140(12):3996-4000. doi: 10.1039/c5an00292c. Epub 2015 Apr 24.
Most electrochemiluminescence (ECL) systems require high concentrations of quencher to totally quench ECL. In this study, we found that ozone can quench tris(2,2'-bipyridyl)ruthenium(II) ECL using formaldehyde, methylglyoxal, or glyoxalate as co-reactants at a glassy carbon electrode with remarkable efficiencies even when the concentration of ozone is merely 0.25% of that of the co-reactant. The strongest quenching is observed with the tris(2,2'-bipyridyl)ruthenium(II)/formaldehyde ECL system. The tris(2,2'-bipyridyl)ruthenium(II)/formaldehyde ECL intensities decrease linearly with the ozone concentration over the range of 0.025-25 μM (r = 0.9947) with a limit of detection of 8 nM. The method is more sensitive and faster than most methods. It shows high selectivity in the presence of other ROS or oxidants and some metal ions, such as H2O2, ClO(-), Mg(2+), Ni(2+), etc. The method exhibits high recoveries for the detection of ozone in a ventilated photocopy room.
大多数电化学发光（ECL）系统需要高浓度的猝灭剂才能完全猝灭ECL。在本研究中，我们发现，以甲醛、甲基乙二醛或乙醛酸酯作为共反应物时，臭氧能够在玻碳电极上高效猝灭三（2,2'-联吡啶）钌（II）的ECL，即便臭氧浓度仅为共反应物浓度的0.25%。在三（2,2'-联吡啶）钌（II）/甲醛ECL体系中观察到最强的猝灭效果。在0.025 - 25 μM范围内，三（2,2'-联吡啶）钌（II）/甲醛的ECL强度随臭氧浓度呈线性下降（r = 0.9947），检测限为8 nM。该方法比大多数方法更灵敏、更快速。在存在其他活性氧或氧化剂以及一些金属离子（如H2O2、ClO(-)、Mg(2+)、Ni(2+)等）的情况下，它显示出高选择性。该方法在通风的复印室中检测臭氧时具有高回收率。
