使用VERSANT HCV基因分型2.0检测法将HCV 2k/1b毒株误分类为HCV 2a/2c的潜在风险。
Potential risk of misclassification HCV 2k/1b strains as HCV 2a/2c using VERSANT HCV Genotype 2.0 assay.
作者信息
De Keukeleire Steven, Descheemaeker Patrick, Reynders Marijke
机构信息
Department of Laboratory Medicine, AZ St-Jan Bruges, Bruges, Belgium.
Department of Laboratory Medicine, AZ St-Jan Bruges, Bruges, Belgium.
出版信息
Diagn Microbiol Infect Dis. 2015 Jul;82(3):201-2. doi: 10.1016/j.diagmicrobio.2015.04.001. Epub 2015 Apr 7.
The performance of a hepatitis C virus (HCV) NS5B sequencing method described by Murphy et al. (Use of sequence analysis of the NS5B region for routine genotyping of hepatitis C virus with reference to C/E1 and 5' untranslated region sequences. J Clin Microbiol 2007;45(4):1102-12.) was compared with the VERSANT HCV Genotype 2.0 assay. The sequencing strategy led to detection of HCV recombinant genotype 2k/1b, previously identified as genotype 2a/2c, which reveals the importance of exact HCV genotyping and subtyping.
将Murphy等人描述的丙型肝炎病毒（HCV）NS5B测序方法的性能（使用NS5B区域的序列分析对丙型肝炎病毒进行常规基因分型，并参考C/E1和5'非翻译区序列。《临床微生物学杂志》2007年；45(4):1102 - 12）与VERSANT HCV基因型2.0检测方法进行了比较。该测序策略导致检测到HCV重组基因型2k/1b，该基因型先前被鉴定为基因型2a/2c，这揭示了精确的HCV基因分型和亚型分型的重要性。
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