血红素前体注射对于通过家蚕表达系统生产分枝状菌过氧化物酶融合蛋白是有效的。
Heme precursor injection is effective for Arthromyces ramosus peroxidase fusion protein production by a silkworm expression system.
作者信息
Hayashi Kounosuke, Lee Jae Man, Tomozoe Yusuke, Kusakabe Takahiro, Kamiya Noriho
机构信息
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan; Hitachi Aloka Medical, Ltd., 6-22-1 Mure, Mitaka-shi, Tokyo 181-8622, Japan.
Laboratory of Silkworm Science, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
出版信息
J Biosci Bioeng. 2015 Oct;120(4):384-6. doi: 10.1016/j.jbiosc.2015.02.013. Epub 2015 Apr 20.
Recombinant peroxidase from Arthromyces ramosus, fused with domains of antibody-binding proteins, was successfully obtained by a silkworm larvae expression system. The catalytic activity of the fusion peroxidase was increased 6-fold with the injection of 5-aminolevulinic acid into silkworm larvae as a heme precursor.
通过家蚕幼虫表达系统成功获得了与抗体结合蛋白结构域融合的来自分枝节丛孢菌的重组过氧化物酶。向家蚕幼虫注射5-氨基乙酰丙酸作为血红素前体后，融合过氧化物酶的催化活性提高了6倍。
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