通过全局表型分析揭示铜绿假单胞菌PAO1的多聚磷酸激酶突变体（ppk1和ppk2）的多种抗生素敏感性
Multiple antibiotic susceptibility of polyphosphate kinase mutants (ppk1 and ppk2) from Pseudomonas aeruginosa PAO1 as revealed by global phenotypic analysis.
作者信息
Ortiz-Severín Javiera, Varas Macarena, Bravo-Toncio Catalina, Guiliani Nicolás, Chávez Francisco P
机构信息
Systems Microbiology Laboratory, Department of Biology, Faculty of Science, University of Chile, Las Palmeras 3425, Ñuñoa, Santiago, Chile.
Bacterial Communication Laboratory, Department of Biology, Faculty of Science, University of Chile, Las Palmeras 3425, Ñuñoa, Santiago, Chile.
出版信息
Biol Res. 2015 Apr 25;48(1):22. doi: 10.1186/s40659-015-0012-0.
BACKGROUND
Pseudomonas aeruginosa is known to be a multidrug resistant opportunistic pathogen. Particularly, P. aeruginosa PAO1 polyphosphate kinase mutant (ppk1) is deficient in motility, quorum sensing, biofilm formation and virulence.
FINDINGS
By using Phenotypic Microarrays (PM) we analyzed near 2000 phenotypes of P. aeruginosa PAO1 polyP kinase mutants (ppk1 and ppk2). We found that both ppk mutants shared most of the phenotypic changes and interestingly many of them related to susceptibility toward numerous and different type of antibiotics such as Ciprofloxacin, Chloramphenicol and Rifampicin.
CONCLUSIONS
Combining the fact that ppk1 mutants have reduced virulence and are more susceptible to antibiotics, polyP synthesis and particularly PPK1, is a good target for the design of molecules with anti-virulence and anti-persistence properties.
背景
铜绿假单胞菌是一种已知的多重耐药机会致病菌。特别是，铜绿假单胞菌PAO1多聚磷酸盐激酶突变体（ppk1）在运动性、群体感应、生物膜形成和毒力方面存在缺陷。
研究结果
通过使用表型微阵列（PM），我们分析了铜绿假单胞菌PAO1多聚P激酶突变体（ppk1和ppk2）的近2000种表型。我们发现这两种ppk突变体共享了大部分表型变化，有趣的是，其中许多变化与对多种不同类型抗生素（如环丙沙星、氯霉素和利福平）的敏感性有关。
结论
结合ppk1突变体毒力降低且更易受抗生素影响这一事实，多聚P合成，尤其是PPK1，是设计具有抗毒力和抗持续性特性分子的良好靶点。
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