Ji Ming-Li, Qian Zhi-Bin, Wu Yun-Hong
Department of physiology, College of Basic Medical Science, Xinxiang Medical University, Xinxiang 453003, China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2015 Apr;35(4):598-601.
To investigate the effects of prenatal alcohol exposure on rhythmic respiratory discharge activity (RRDA) in the medullary slices of neonatal rats.
Ten pregnant female SD rats were exposed to 0, 4%, 6%, 8%, and 10% alcohol in drinking water from 1 week before till 3 days after delivery. The medullary slices of the neonatal rats containing the medial region of the nucleus retrofacialis (mNRF) with the hypoglossal nerve rootlets were prepared and perfused with modified Kreb's solution to record RRDA from the hypoglossal nerve rootlets using suction electrodes.
No significant difference was found in RRDA in 50 min among the neonatal rats with prenatal exposure to 0, 4%, 6%, and 8% alcohol, but the RRDA in 10% alcohol exposure group became irregular. Prenatal exposure to increased alcohol concentrations caused attenuated RRDA attenuated in the neonatal rats, shown by shortened inspiratory time (TI), decreased respiratory frequency (RF), and reduced integral amplitude (IA) as compared with those in the control group.
Prenatal alcohol exposure inhibits RRDA in medullary slices of neonatal rats, which might be a mechanism by which maternal alcohol exposure causes suppressed offspring respiratory functions.
研究产前酒精暴露对新生大鼠延髓切片中节律性呼吸放电活动（RRDA）的影响。
10只怀孕的雌性SD大鼠在分娩前1周直至分娩后3天，饮用含0%、4%、6%、8%和10%酒精的水。制备含有面神经后核内侧区域（mNRF）和舌下神经根的新生大鼠延髓切片，并用改良的克雷布斯溶液灌注，使用吸力电极从舌下神经根记录RRDA。
产前暴露于0%、4%、6%和8%酒精的新生大鼠在50分钟内RRDA无显著差异，但10%酒精暴露组的RRDA变得不规则。与对照组相比，产前暴露于浓度增加的酒精会导致新生大鼠RRDA减弱，表现为吸气时间（TI）缩短、呼吸频率（RF）降低和积分幅度（IA）减小。
产前酒精暴露抑制新生大鼠延髓切片中的RRDA，这可能是母体酒精暴露导致后代呼吸功能受抑制的一种机制。
