Coveñas R, Alonso J R, Conrath M
Facultad de Biología, Dpto. Citología, Salamanca, Spain.
Brain Res Bull. 1989 Oct-Nov;23(4-5):277-81. doi: 10.1016/0361-9230(89)90209-8.
The distribution of enkephalin-like cell bodies in the thalamus of the rat was studied by means of intratissular injections of colchicine and using an indirect immunoperoxidase technique. The densest clusters of immunoreactive perikarya were observed in the nuclei geniculatum lateralis ventralis, medialis dorsalis, centralis lateralis, centralis medialis and anterior ventralis. Whereas the nuclei praetectalis lateralis, lateralis posterior, habenularis lateralis, parataenialis (its caudal part), parafascicularis, centrum medianum, reuniens and ventralis medialis had the lowest density. In other thalamic nuclei geniculatum mediale, paraventricularis and parataenialis (its rostral part) the density of enkephalin-like cell bodies was intermediate. These results suggest that the intratissular injection of colchicine is the better way of administration of the drug in order to study the distribution of peptidergic cell bodies in the mammalian CNS. The similarities and differences found in the distribution of enkephalinergic cell populations in the thalamus of different mammals are discussed.
通过向大鼠丘脑组织内注射秋水仙碱并采用间接免疫过氧化物酶技术，研究了脑啡肽样细胞体在大鼠丘脑中的分布。在外侧膝状体腹侧核、背内侧核、外侧中央核、内侧中央核和腹前核中观察到免疫反应性核周体最密集的簇。而外侧前视核、后外侧核、外侧缰核、旁缰核（其尾部）、束旁核、中央中核、连合核和内侧腹核的密度最低。在其他丘脑核团，如内侧膝状体、室旁核和旁缰核（其头部），脑啡肽样细胞体的密度处于中等水平。这些结果表明，为了研究肽能细胞体在哺乳动物中枢神经系统中的分布，向组织内注射秋水仙碱是更好的给药方式。文中还讨论了在不同哺乳动物丘脑中脑啡肽能细胞群分布中发现的异同。
