Goodale Lindsay, Frank Nicholas, Hermida Pilar, D'Oench Sarah
Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA 01536.
Am J Vet Res. 2015 May;76(5):437-44. doi: 10.2460/ajvr.76.5.437.
To determine whether plasma ACTH concentrations vary following administration of a thyrotropin-releasing hormone (TRH) solution prepared for research purposes and stored at -20°C (rTRH) or prepared by a compounding pharmacy and stored at room temperature (approx 22°C; cTRH).
34 adult horses.
The study consisted of 2 experiments. In experiment 1, each horse underwent 2 TRH stimulation tests separated by 24 hours; 10 horses were administered cTRH for the first test and rTRH for the second test (group 1), 10 horses were administered rTRH for the first test and cTRH for the second test (group 2), and 10 horses were administered rTRH for both tests (group 3). Plasma ACTH concentrations were measured at 0 (baseline) and 30 minutes after TRH administration and the delta ACTH responses (change in ACTH concentration after TRH administration) were calculated. In experiment 2, the design was the same as that for experiment 1 except there were 14 days between tests, ACTH was measured at 0 and 10 minutes after TRH administration, and 11, 9, and 10 horses were assigned to groups 1, 2, and 3, respectively.
Adverse effects associated with TRH administration included transient coughing and yawning. In experiment 1, the median delta ACTH response for the second test was significantly lower than that for the first test for all groups. In experiment 2, the median delta ACTH response did not differ significantly between the first and second tests for any group, ACTH concentrations after rTRH administration were positively correlated (rs = 0.95) with those after cTRH administration, and the mean ± SD bias in post-TRH ACTH concentration between rTRH and cTRH was 2.9 ± 12.4 pg/mL.
Results indicated that the TRH stimulation test should not be repeated within 24 hours, and cTRH solution stored at room temperature could be used to effectively perform TRH stimulation testing in horses.
确定给予为研究目的制备并储存于-20°C的促甲状腺激素释放激素（TRH）溶液（rTRH）或由配制药房制备并储存于室温（约22°C；cTRH）后，血浆促肾上腺皮质激素（ACTH）浓度是否会发生变化。
34匹成年马。
该研究包括2个实验。在实验1中，每匹马接受2次间隔24小时的TRH刺激试验；10匹马在第一次试验中给予cTRH，第二次试验中给予rTRH（第1组），10匹马在第一次试验中给予rTRH，第二次试验中给予cTRH（第2组），10匹马在两次试验中均给予rTRH（第3组）。在给予TRH后0（基线）和30分钟测量血浆ACTH浓度，并计算ACTH的变化反应（给予TRH后ACTH浓度的变化）。在实验2中，设计与实验1相同，只是试验间隔为14天，在给予TRH后0和10分钟测量ACTH，分别有11、9和10匹马被分配到第1、2和3组。
与给予TRH相关的不良反应包括短暂咳嗽和打哈欠。在实验1中，所有组第二次试验的ACTH变化反应中位数均显著低于第一次试验。在实验2中，任何组第一次和第二次试验的ACTH变化反应中位数均无显著差异，给予rTRH后的ACTH浓度与给予cTRH后的ACTH浓度呈正相关（rs = 0.95），rTRH和cTRH之间给予TRH后ACTH浓度的平均±标准差偏差为2.9±12.4 pg/mL。
结果表明，TRH刺激试验不应在24小时内重复进行，储存于室温的cTRH溶液可有效用于马的TRH刺激试验。
