Lotfy Hayam M, Hegazy Maha A, Rezk Mamdouh R, Omran Yasmin Rostom
Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr el Aini Street, 11562 Cairo, Egypt; Pharmaceutical Chemistry Department, Faculty of Pharmaceutical Sciences & Pharmaceutical Industries, Future University, 12311 Cairo, Egypt.
Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr el Aini Street, 11562 Cairo, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Sep 5;148:328-37. doi: 10.1016/j.saa.2015.04.004. Epub 2015 Apr 8.
Smart spectrophotometric methods have been applied and validated for the simultaneous determination of a binary mixture of chloramphenicol (CPL) and prednisolone acetate (PA) without preliminary separation. Two novel methods have been developed; the first method depends upon advanced absorbance subtraction (AAS), while the other method relies on advanced amplitude modulation (AAM); in addition to the well established dual wavelength (DW), ratio difference (RD) and constant center coupled with spectrum subtraction (CC-SS) methods. Accuracy, precision and linearity ranges of these methods were determined. Moreover, selectivity was assessed by analyzing synthetic mixtures of both drugs. The proposed methods were successfully applied to the assay of drugs in their pharmaceutical formulations. No interference was observed from common additives and the validity of the methods was tested. The obtained results have been statistically compared to that of official spectrophotometric methods to give a conclusion that there is no significant difference between the proposed methods and the official ones with respect to accuracy and precision.
智能分光光度法已被应用并验证用于不经预分离同时测定氯霉素（CPL）和醋酸泼尼松龙（PA）的二元混合物。已开发出两种新方法；第一种方法基于先进吸光度减法（AAS），而另一种方法依赖于先进幅度调制（AAM）；此外还有成熟的双波长（DW）、比率差（RD）和恒定中心结合光谱减法（CC - SS）方法。测定了这些方法的准确度、精密度和线性范围。此外，通过分析两种药物的合成混合物评估了选择性。所提出的方法成功应用于药物制剂中药物的测定。未观察到常见添加剂的干扰，并测试了方法的有效性。将所得结果与官方分光光度法进行了统计学比较，得出结论：在所提出的方法与官方方法之间，在准确度和精密度方面没有显著差异。
