Thomassen D G, Chen B T, Mauderly J L, Johnson N F, Griffith W C
Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute, Albuquerque, NM 87185.
Carcinogenesis. 1989 Dec;10(12):2359-61. doi: 10.1093/carcin/10.12.2359.
Rats were exposed 6 h/day, 5 days/week for 5 weeks to levels of cigarette smoke designed to deposit amounts of particulate per gram of lung similar to those experienced by a heavy smoker. Tracheal epithelial cells were isolated and examined in culture for the presence of preneoplastic variants. Two- to three-fold increases in the frequency of variants were observed in cells isolated from smoke-exposed animals compared to those from air-exposed controls. These results provide an estimate of the frequency of cigarette smoke-induced initiation events in respiratory epithelium that will be useful in further defining the role of cigarette smoke in the development of lung cancer.
大鼠每天暴露于香烟烟雾中6小时，每周5天，持续5周，所接触的香烟烟雾水平旨在使每克肺组织沉积的颗粒量与重度吸烟者所经历的相似。分离气管上皮细胞并在培养中检查是否存在癌前变体。与空气暴露对照组相比，在从烟雾暴露动物分离的细胞中观察到变体频率增加了两到三倍。这些结果提供了香烟烟雾诱导的呼吸道上皮起始事件频率的估计值，这将有助于进一步确定香烟烟雾在肺癌发生中的作用。
