Prasertsincharoen Noppadol, Constantinoiu Constantin, Gardiner Christopher, Warner Jeffrey, Elliman Jennifer
Biomedicine, College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, Queensland, Australia Veterinary Technology, Kasetsart University, Bangkok, Thailand
Biomedicine, College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, Queensland, Australia.
Appl Environ Microbiol. 2015 Jul;81(13):4368-75. doi: 10.1128/AEM.00317-15. Epub 2015 Apr 24.
Burkholderia pseudomallei is a saprophytic bacterium that causes melioidosis and is often isolated from rice fields in Southeast Asia, where the infection incidence is high among rice field workers. The aim of this study was to investigate the relationship between this bacterium and rice through growth experiments where the effect of colonization of domestic rice (Oryza sativa L. cv Amaroo) roots by B. pseudomallei could be observed. When B. pseudomallei was exposed to surface-sterilized seeds, the growth of both the root and the aerosphere was retarded compared to that in controls. The organism was found to localize in the root hairs and endodermis of the plant. A biofilm formed around the root and root structures that were colonized. Growth experiments with a wild rice species (Oryza meridionalis) produced similar retardation of growth, while another domestic cultivar (O. sativa L. cv Koshihikari) did not show retarded growth. Here we report B. pseudomallei infection and inhibition of O. sativa L. cv Amaroo, which might provide insights into plant interactions with this important human pathogen.
类鼻疽伯克霍尔德菌是一种腐生细菌，可引起类鼻疽，常从东南亚的稻田中分离出来，在那里稻田工人的感染发病率很高。本研究的目的是通过生长实验来研究这种细菌与水稻之间的关系，在该实验中可以观察到类鼻疽伯克霍尔德菌对国产水稻（Oryza sativa L. cv Amaroo）根部的定殖影响。当类鼻疽伯克霍尔德菌接触表面灭菌的种子时，与对照组相比，根和空气层的生长均受到抑制。发现该生物体定位于植物的根毛和内皮层中。在被定殖的根和根结构周围形成了生物膜。对一种野生稻品种（Oryza meridionalis）进行的生长实验产生了类似的生长抑制，而另一个国产栽培品种（O. sativa L. cv Koshihikari）则未表现出生长受抑制的情况。在此我们报告了类鼻疽伯克霍尔德菌对O. sativa L. cv Amaroo的感染和抑制作用，这可能为了解植物与这种重要的人类病原体之间的相互作用提供见解。
