Voloshchuk O N, Kopyl'chuk G P, Buchkovskaia I M
Eksp Klin Gastroenterol. 2014(8):96-100.
The activity of the sorbitoldehydrogenase (SDH), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) in the blood serum of rats with acetaminophen-induced hepatitis under the conditions of alimentary deprivation of protein was studied. The animals were divided into 3 groups: 1--rats with acute acetaminophen-induced hepatitis, maintained on the full ration; 2--rats with acute acetaminophen-induced hepatitis, maintained under the conditions of alimentary deprivation of protein; 3--control. The activity of the sorbitol dehydrogenase in blood serum was determined by the kinetic method, activity of the alanine aminotransferase and alkaline phosphatase - photometrically. It is shown, that in animals with the model hepatitis the activity of sorbitol dehydrogenase in blood serum increases 20-fold, wherein statistical significance between animals with hepatitis maintained under the conditions of full ration and those of low-protein diet is not established. In the group of animals with acetaminophen-induced hepatitis the preservation on the control level of the alkaline phosphatase activity on the base of the increase of alanine aminotransferase by 2.2 times and ratio ALT/ALP>5 testifies about hepatocellular liver injury. In the group of animals with drug-induced hepatitis and alimentary deprivation of protein, the increase of the alkaline phosphatase and alanine aminotransferase activity is observed, herewith the ratio ALT/ALP ranges from 2 to 5 and testifies about mixed liver injury. The conclusion was made, that alimentary deprivation of protein is the critical factor for the development of the disturbances of functional and structural liver integrity, and the therapeutic approaches to the correction of the drug-induced liver injury should be different depending on the value of protein ration in the anamnesis, taking into account the different types of liver injury.
研究了在蛋白质营养缺乏条件下，对乙酰氨基酚诱导肝炎大鼠血清中山梨醇脱氢酶（SDH）、丙氨酸转氨酶（ALT）和碱性磷酸酶（ALP）的活性。将动物分为3组：1组为对乙酰氨基酚诱导急性肝炎且维持全量饮食的大鼠；2组为对乙酰氨基酚诱导急性肝炎且在蛋白质营养缺乏条件下维持的大鼠；3组为对照组。血清中山梨醇脱氢酶的活性采用动力学方法测定，丙氨酸转氨酶和碱性磷酸酶的活性采用比色法测定。结果表明，在模型肝炎动物中，血清中山梨醇脱氢酶的活性增加了20倍，其中在全量饮食条件下维持的肝炎动物与低蛋白饮食的肝炎动物之间未发现统计学差异。在对乙酰氨基酚诱导肝炎的动物组中，碱性磷酸酶活性维持在对照水平，而丙氨酸转氨酶增加2.2倍且ALT/ALP比值>5，这证明存在肝细胞性肝损伤。在药物诱导肝炎且蛋白质营养缺乏的动物组中，观察到碱性磷酸酶和丙氨酸转氨酶活性增加，此时ALT/ALP比值在2至5之间，证明存在混合性肝损伤。得出的结论是，蛋白质营养缺乏是肝脏功能和结构完整性紊乱发展的关键因素，针对药物性肝损伤的治疗方法应根据既往病史中蛋白质摄入量的不同而有所不同，同时要考虑到不同类型的肝损伤。
