Savlı Hakan, Akkoyunlu Ramis Ufuk, Çine Naci, Gluzman Daniil F, Zavelevich Michael P, Sklyarenko Lilia M, Koval Stella V, Sünnetçi Deniz
Kocaeli University Faculty of Medicine, Department of Medical Genetics, Kocaeli, Turkey. E-mail :
Turk J Haematol. 2016 Mar 5;33(1):8-14. doi: 10.4274/tjh.2014.0190. Epub 2015 Apr 27.
Nuclear factor kappa B (NF-κB) is an important transcription factor in cancer and NF-κB activation has been seen in angiogenesis, tumor progression, and metastasis. Relationships between specific NF-κB gene networks, leukemogenesis, and radiation exposure are still unknown. Our aim was to study the expression levels of the NF-κB1, NF-κB2, and Rel genes in hematological malignancies in the post-Chernobyl period.
We analyzed gene expression levels of NF-κB1, NF-κB2, and Rel in 49 B-cell chronic lymphocytic leukemia, 8 B-cell non-Hodgkin's lymphoma, 3 acute myeloid leukemia, 3 chronic myeloid leukemia, 2 hairy cell leukemia, 2 myelodysplastic syndrome, and 2 T-cell large granular lymphocytic leukemia patients using real-time polymerase chain reaction.
Expression levels of NF-κB1, NF-κB2, and Rel genes were found to be deregulated.
These results could be accepted as specific gene traces to radiation-induced leukemia or as potential candidates for new diagnostic biomarker studies. Larger experiments and non-exposed control malignant cell populations are needed to clarify these suggestions.
核因子κB（NF-κB）是癌症中一种重要的转录因子，且在血管生成、肿瘤进展和转移过程中均可见NF-κB激活。特定的NF-κB基因网络、白血病发生与辐射暴露之间的关系仍不清楚。我们的目的是研究切尔诺贝利事故后时期血液系统恶性肿瘤中NF-κB1、NF-κB2和Rel基因的表达水平。
我们采用实时聚合酶链反应分析了49例B细胞慢性淋巴细胞白血病、8例B细胞非霍奇金淋巴瘤、3例急性髓系白血病、3例慢性髓系白血病、2例毛细胞白血病、2例骨髓增生异常综合征和2例T细胞大颗粒淋巴细胞白血病患者中NF-κB1、NF-κB2和Rel的基因表达水平。
发现NF-κB1、NF-κB2和Rel基因的表达水平失调。
这些结果可被视为辐射诱导白血病的特定基因痕迹，或作为新诊断生物标志物研究的潜在候选物。需要进行更大规模的实验以及未暴露的对照恶性细胞群体来阐明这些推测。
