Kimura Koichi, Daimon Masao, Morita Hiroyuki, Kawata Takayuki, Nakao Tomoko, Okano Tomoko, Lee Seitetsu L, Takenaka Katsu, Nagai Ryozo, Yatomi Yutaka, Komuro Issei
Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo.
Int Heart J. 2015 May 13;56(3):349-53. doi: 10.1536/ihj.14-367. Epub 2015 Apr 27.
Speckle tracking echocardiography (STE) has been reported to be a promising technique for evaluating right ventricular (RV) function in the clinical setting. On the other hand, the usefulness of STE for RV evaluation in small animal models has not been clarified, although the rat model is among the most commonly used animal models to develop novel effective treatments against pulmonary hypertension and RV heart failure (HF).We validated the use of STE and conventional echocardiographic variables for evaluating RV functions in a rat model by comparing the echocardiographic values of RVHF rats (n = 12) induced by monocrotaline injection with those of control rats (n = 12).Most conventional echocardiographic variables demonstrated that RVHF rats have significant RV dysfunction. The area under the curve (AUC) values to distinguish RV dysfunction in RVHF rats from normal RV function in control rats using fractional area change (FAC), tricuspid annular plane systolic excursion (TAPSE), RV myocardial performance index (MPI), peak tissue Doppler tricuspid annular velocities at systole (Sa), and at early diastole (Ea) were 0.71, 0.98, 0.79, 0.92, and 0.91, respectively. However, using STE analysis for RV evaluation, limited reproducibility was observed (variability 19-37 %, ICC 0.74-0.88) and the only circumferential strain showed significantly lower absolute values (P = 0.039, AUC = 0.76).To evaluate RV function in rat models, circumferential strain may be useful, however, the reproducibility and diagnostic utility were limited. Conventional echocardiographic variables such as TAPSE, tissue Doppler Sa, and Ea have superior diagnostic utility.
斑点追踪超声心动图（STE）据报道是一种在临床环境中评估右心室（RV）功能的有前景的技术。另一方面，尽管大鼠模型是开发针对肺动脉高压和右心室心力衰竭（HF）的新型有效治疗方法最常用的动物模型之一，但STE在小动物模型中评估RV的实用性尚未明确。我们通过比较用野百合碱注射诱导的RVHF大鼠（n = 12）与对照大鼠（n = 12）的超声心动图值，验证了STE和传统超声心动图变量在大鼠模型中评估RV功能的用途。大多数传统超声心动图变量表明RVHF大鼠存在明显的RV功能障碍。使用面积变化分数（FAC）、三尖瓣环平面收缩期位移（TAPSE）、右心室心肌性能指数（MPI）、收缩期三尖瓣环组织多普勒峰值速度（Sa）和舒张早期（Ea）来区分RVHF大鼠的RV功能障碍与对照大鼠的正常RV功能的曲线下面积（AUC）值分别为0.71、0.98、0.79、0.92和0.91。然而，使用STE分析评估RV时，观察到再现性有限（变异性19 - 37%，组内相关系数0.74 - 0.88），并且只有圆周应变显示出明显较低的绝对值（P = 0.039，AUC = 0.76）。为了评估大鼠模型中的RV功能，圆周应变可能有用，然而，其再现性和诊断效用有限。传统超声心动图变量如TAPSE、组织多普勒Sa和Ea具有更高的诊断效用。
