Eze J I, Ayogu L C, Abonyi F O, Eze U U
Department of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria.
Department of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria.
Exp Parasitol. 2015 Jul;154:87-92. doi: 10.1016/j.exppara.2015.04.015. Epub 2015 Apr 23.
Zinc is an essential trace element crucial for normal development and function of cells mediating nonspecific immunity and protects bio-molecules from oxidative damage. This study was designed to assess the effects of dietary zinc supplementation on anaemia and immunity of trypanosome-infected rats. Thirty rats, divided into five groups (A-E) of 6 each, were used for the study. Parameters used to assess the effect of the supplementation are antibody response to Sheep RBC using direct haemagglutination test, parasitaemia using the rapid matching method, WBC count using the improved Neubauer haemocytometer method, haemoglobin concentration using the cynomethaemoglobin technique while PCV was determined using the microhaematocrit method. The pre-infection supplementation did not prolong the pre-patent period significantly (p > 0.05). However, it significantly (p < 0.05) increased the packed cell volume (PCV), haemoglobin (Hb) concentration, leucocyte count, and antibody titre by day 7 on the supplementation (OTS). Following infection on day 7 OTS, the PCV and Hb decreased but remained significantly (p < 0.05) higher than the infected not supplemented (INS) group, while on day 14 OTS, they maintained a significantly (p < 0.05) higher antibody titre as compared to other groups. On day 21 OTS, theweight of 8 ppm and not infected not supplemented (NINS) groups was significantly (p < 0.05) higher but the relative organ weight of their liver and spleen was significantly (p < 0.05) lower than 2 ppm, 4 ppm and INS groups. On day 21 OTS, the parasitaemia levels of INS group was significantly (p < 0.05) higher than the supplemented groups. From the results, dietary zinc supplementation can be useful in the management of anaemia and immunosupression caused by trypanosomes in rats.
锌是一种必需的微量元素，对介导非特异性免疫的细胞的正常发育和功能至关重要，并能保护生物分子免受氧化损伤。本研究旨在评估日粮中补充锌对感染锥虫大鼠贫血和免疫的影响。将30只大鼠分为5组（A - E），每组6只，用于本研究。用于评估补充效果的参数包括：采用直接血凝试验检测对绵羊红细胞的抗体反应、采用快速匹配法检测虫血症、采用改良的纽鲍尔血细胞计数板法检测白细胞计数、采用氰化高铁血红蛋白技术检测血红蛋白浓度，同时采用微量血细胞比容法测定血细胞比容。感染前补充锌并未显著延长潜伏期（p > 0.05）。然而，在补充锌7天后（OTS），它显著（p < 0.05）增加了血细胞比容（PCV）、血红蛋白（Hb）浓度、白细胞计数和抗体滴度。在OTS第7天感染后，PCV和Hb下降，但仍显著（p < 0.05）高于未补充锌的感染组（INS），而在OTS第14天，与其他组相比，它们的抗体滴度仍显著（p < 0.05）更高。在OTS第21天，8 ppm且未感染未补充锌（NINS）组的体重显著（p < 0.05）更高，但其肝脏和脾脏的相对器官重量显著（p < 0.05）低于2 ppm、4 ppm和INS组。在OTS第21天，INS组的虫血症水平显著（p < 0.05）高于补充锌的组。从结果来看，日粮中补充锌可有助于管理大鼠锥虫感染引起的贫血和免疫抑制。
