El-Dib Nadia A, El-Badry Ayman A, Ta-Tang Thuy-Huong, Rubio Jose M
Department of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Egypt.
Department of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Egypt.
Exp Parasitol. 2015 Jul;154:127-33. doi: 10.1016/j.exppara.2015.04.011. Epub 2015 Apr 23.
Human infection with Capillaria philippinensis is accidental; however, it may end fatally if not diagnosed and treated in the proper time. The first case was detected in the Philippines in 1963, but later reported in other countries around the world, including Egypt. In this report, molecular diagnosis using a specific nested PCR for detection of C. philippinensis in faeces is described based on the amplification of small ribosomal subunit. The test showed sensitivity and specificity, as it detected all the positive cases and gave no cross-reaction with human DNA and DNA of other tested parasites. This method can be very useful not only for improvement of diagnosis, but also to understand the different environmental routes of transmission by detection of C. philippinensis DNA-stages in the possible fish intermediate hosts and reservoir animal host, helping to improve strategies for surveillance and prevention of human disease.
人体感染菲律宾毛细线虫是偶然发生的；然而，如果没有在适当时间进行诊断和治疗，可能会导致致命后果。1963年在菲律宾发现了首例病例，但后来在世界其他国家也有报告，包括埃及。在本报告中，描述了一种基于小核糖体亚基扩增的特异性巢式PCR用于检测粪便中菲律宾毛细线虫的分子诊断方法。该检测显示出敏感性和特异性，因为它检测到了所有阳性病例，并且与人类DNA和其他测试寄生虫的DNA没有交叉反应。这种方法不仅对改善诊断非常有用，而且通过检测可能的鱼类中间宿主和储存动物宿主中的菲律宾毛细线虫DNA阶段，有助于了解不同的环境传播途径，从而有助于改进人类疾病的监测和预防策略。
