Awobusuyi Temitope D, Siwela Muthulisi, Kolanisi Unathi, Amonsou Eric O
Department of Biotechnology and Food Technology, Durban University of Technology, Durban, South Africa.
School of Agricultural, Earth and Environmental Sciences, University of KwaZulu-Natal, KwaZulu-Natal, South Africa.
J Sci Food Agric. 2016 Mar 15;96(4):1356-61. doi: 10.1002/jsfa.7230. Epub 2015 Jun 3.
Vitamin A deficiency is a major public health problem in sub-Saharan Africa. Amahewu is a popular southern African lactic acid fermented non-alcoholic maize-based beverage, which is deficient in vitamin A. In this study, provitamin A retention and sensory acceptability of amahewu processed using provitamin A-biofortified maize and three types of inoculums during fermentation (malted maize, wheat bran and Lactobacillus starter culture) were investigated.
The total provitamin A content of amahewu samples, estimated as β-carotene, β-cryptoxanthin and α-carotene content, ranged from 3.3 to 3.8 g kg(-1) (dry weight). Provitamin A was substantially retained (79- 90% β-carotene equivalent) in amahewu after fermentation. Amahewu samples prepared with added starter cultures had the lowest retention of provitamin A. Consumers (approx. 69%) liked provitamin A-biofortified amahewu either moderately or very much. Principal component analysis of amahewu sensory data showed that 71% of variation was due to maize types and 18% of variation could be due to the inoculum used during fermentation. Amahewu samples prepared using provitamin A-biofortified maize were slightly more liked (mean score: 7.0 ± 1.2) compared to those of white maize reference samples. The use of starter culture combined with either malted maize or wheat bran as inoculum during fermentation improved the taste and aroma of amahewu and hence its acceptability.
Provitamin A is substantially retained in amahewu after fermentation. The slightly high acceptability of amahewu prepared using provitamin A-biofortified maize compared to that of white maize thus suggests that fermented product like amahewu can potentially be used to deliver provitamin A to vulnerable individuals.
维生素A缺乏是撒哈拉以南非洲地区的一个主要公共卫生问题。阿玛赫武是一种在南部非洲很受欢迎的以玉米为原料的乳酸发酵非酒精饮料,其维生素A含量较低。在本研究中,对使用富含维生素A原的生物强化玉米和三种发酵接种物(发芽玉米、麦麸和乳酸菌发酵剂)加工的阿玛赫武中维生素A原的保留情况和感官可接受性进行了研究。
以β-胡萝卜素、β-隐黄质和α-胡萝卜素含量估算,阿玛赫武样品中的总维生素A原含量在3.3至3.8克/千克(干重)之间。发酵后,阿玛赫武中维生素A原大量保留(β-胡萝卜素当量的79%-90%)。添加发酵剂制备的阿玛赫武样品中维生素A原的保留率最低。约69%的消费者对富含维生素A原的生物强化阿玛赫武中度或非常喜欢。阿玛赫武感官数据的主成分分析表明,71%的变异归因于玉米类型,18%的变异可能归因于发酵过程中使用的接种物。与白玉米参考样品相比,使用富含维生素A原的生物强化玉米制备的阿玛赫武样品略更受欢迎(平均得分:7.0±1.2)。在发酵过程中使用发酵剂与发芽玉米或麦麸作为接种物相结合,改善了阿玛赫武的口感和香气,从而提高了其可接受性。
发酵后阿玛赫武中维生素A原大量保留。与白玉米相比,使用富含维生素A原的生物强化玉米制备的阿玛赫武的可接受性略高,这表明像阿玛赫武这样的发酵产品有可能用于向易受影响的个体提供维生素A原。