Bouchez D, Tokuhisa J G, Llewellyn D J, Dennis E S, Ellis J G
CSIRO Division of Plant Industry, Canberra, ACT, Australia.
EMBO J. 1989 Dec 20;8(13):4197-204. doi: 10.1002/j.1460-2075.1989.tb08605.x.
The ocs-element is an enhancer element first identified in the promoter of the octopine synthase gene (OCS) where it occurs as a 16 bp palindromic sequence. The transcriptional enhancing activity of the ocs-element correlated with in vitro binding of a transcription factor. We have now identified ocs-elements in the promoter regions of six other T-DNA genes involved in opine synthesis and three plant viral promoters including the 35S promoter of cauliflower mosaic virus. These elements bind the ocs transcription factor in vitro and enhance transcription in plant cells. Comparison of the sequences of these 10 elements has defined a 20 bp consensus sequence, TGACG(T/C)AAG(C/G)(G/A)(A/C)T(G/T)ACG(T/C)(A/C)(A/C), which includes the 16 bp palindrome in its central region. We propose the name ocs-element for this class of promoter elements of similar sequence and function.
ocs元件是一种增强子元件,最初在章鱼碱合成酶基因(OCS)的启动子中被鉴定出来,它以16bp的回文序列形式出现。ocs元件的转录增强活性与一种转录因子的体外结合相关。我们现已在参与冠瘿碱合成的其他六个T-DNA基因以及三个植物病毒启动子(包括花椰菜花叶病毒的35S启动子)的启动子区域中鉴定出ocs元件。这些元件在体外与ocs转录因子结合,并增强植物细胞中的转录。对这10个元件的序列进行比较,确定了一个20bp的共有序列,即TGACG(T/C)AAG(C/G)(G/A)(A/C)T(G/T)ACG(T/C)(A/C)(A/C),其中心区域包含16bp的回文序列。我们为这类具有相似序列和功能的启动子元件提议命名为ocs元件。