Doughty M J
University of Waterloo, School of Optometry, Ontario, Canada.
Exp Eye Res. 1989 Nov;49(5):807-27. doi: 10.1016/s0014-4835(89)80041-7.
Eyes from female albino rabbits (1.9-2.3 kg) were enucleated immediately following euthanasia along with the lids and conjunctiva. The globes were moist chamber-stored (in a 4 degrees C refrigerator with the lids closed and the cornea facing downwards) for 12-500 hr. Central corneal thickness (by ultrasound) increased from 350 to approximately 650 microns within 72 hr but changed little thereafter. In the latter period, the relative fluid pressure of the globe (pneumatonography) dropped to less than 10 mmHg, aqueous bicarbonate (tCO2) levels fell to less than 5 mM and anterior chamber fluid tonicity decreased progressively (especially after 24 hr storage) to reach values of around 200 mosmol l-1 by 7 days. Storma-endothelium preparations of the corneas, after 2 hr of in vitro equilibration with a bicarbonate-Ringer solution (supplemented with glucose, adenosine and glutathione) were evaluated for their ability to undergo deturgescence under silicone oil or to pump fluid against a hydrostatic pressure of 20 cmH2O. Corneal preparations from up to 7 days storage showed rapid (60 to 135 microns hr-1) and complete deturgescence (net change in thickness of 140-180 microns) that was maintained. Thereafter, the ability to show deturgescence declined to zero by 10 days. In marked contrast, endothelial fluid pump activity (of approximately 5 microliters hr-1) was manifest for only 36 hr after which time this function rapidly declined. Most corneas stored for periods longer than 48 hr exhibited a continuous leak (of -1 to -5 microliters hr-1). The results indicate that corneal deturgescence and endothelial fluid pump function are not necessarily coupled in vitro.
对体重为1.9 - 2.3千克的雌性白化兔实施安乐死后，立即摘除其眼球，连带眼睑和结膜一起取下。将眼球置于湿盒中保存（在4℃冰箱中，盖上眼睑，角膜朝下）12 - 500小时。中央角膜厚度（通过超声测量）在72小时内从350微米增加到约650微米，但此后变化不大。在后期，眼球的相对流体压力（眼压描记法）降至低于10 mmHg，房水碳酸氢盐（tCO2）水平降至低于5 mM，前房液张力逐渐降低（尤其是在储存24小时后），到7天时达到约200 mosmol l-1的值。角膜的基质 - 内皮制剂在与碳酸氢盐 - 林格溶液（补充有葡萄糖、腺苷和谷胱甘肽）进行2小时的体外平衡后，评估其在硅油下消肿的能力或抵抗20 cmH2O静水压力泵出液体的能力。储存长达7天的角膜制剂显示出快速（60至135微米/小时）且完全的消肿（厚度净变化为140 - 180微米），并能维持该状态。此后，消肿能力在10天时降至零。形成鲜明对比的是，内皮液泵活性（约为5微升/小时）仅在36小时内表现出来，此后该功能迅速下降。大多数储存超过48小时的角膜表现出持续渗漏（-1至-5微升/小时）。结果表明，角膜消肿和内皮液泵功能在体外不一定相关联。
