Vlasov V V, Rykova E Iu, Ponomareva A A, Zaporozhchenko I A, Morozkin E S, Cherdyntseva N V, Laktionov P P
Mol Biol (Mosk). 2015 Jan-Feb;49(1):55-66.
The major methods of microRNA extraction from different biological fluids (particularly, serum and plasma), approaches to the analysis of microRNA concentration and composition, normalization methods used in data analysis are outlined in the review. The advantages and disadvantages of the described methodological approaches are being highlighted. Special attention is given to microRNAs, circulating in blood, which could be used as the markers for minimally invasive lung cancer diagnostics, prediction of antitumor treatment efficiency and disease prognosis. Prospects and limitations arising from the evaluation of clinical significance of microRNAs as the potential tumor markers, and emerging as roles of various microRNAs in the pathogenesis of lung cancer become known, are discussed.
该综述概述了从不同生物流体（尤其是血清和血浆）中提取微小RNA的主要方法、微小RNA浓度和组成的分析方法以及数据分析中使用的标准化方法。文中强调了所述方法的优缺点。特别关注血液中循环的微小RNA，其可作为微创肺癌诊断、抗肿瘤治疗效果预测和疾病预后的标志物。讨论了评估微小RNA作为潜在肿瘤标志物的临床意义所产生的前景和局限性，以及各种微小RNA在肺癌发病机制中所起作用的相关情况。
