Sokolenko G G, Karpechenko N A
Mikrobiologiia. 2015 Jan-Feb;84(1):37-42.
Expression of the genes encoding the enzymes involved in inulin, sucrose, and glucose metabolism in the yeasts Saccharomyces cerevisiae and Kluyveromyces marxianus was studied. The exon-intron structure of the relevant genes was identified and the primers for quantitative PCR were optimized. Expression of the genes was found to depend on the carbon source. Glucose was shown to exhibit a repressive effect on inulinase synthesis by K. marxianus, while in S. cerevisiae glucose and sucrose were inulinase inducer and repressor, respectively.
研究了酿酒酵母和马克斯克鲁维酵母中参与菊粉、蔗糖和葡萄糖代谢的酶的编码基因的表达。确定了相关基因的外显子-内含子结构，并优化了定量PCR引物。发现这些基因的表达取决于碳源。结果表明，葡萄糖对马克斯克鲁维酵母的菊粉酶合成具有抑制作用，而在酿酒酵母中，葡萄糖和蔗糖分别是菊粉酶的诱导剂和抑制剂。
