Guangzhou Institute of Energy Conversion, Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, China.
Guangzhou Institute of Energy Conversion, Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, China.
Bioresour Technol. 2015 Aug;190:7-12. doi: 10.1016/j.biortech.2015.04.059. Epub 2015 Apr 21.
In the process of liquid hot water (LHW) pretreatment, there are numbers of pellets formed on the lignocellulosic surface. The characteristics and effect of pellets on the enzymatic hydrolysis of LHW-treated sugarcane bagasse (SCB) were investigated. After SCB was treated with LHW at 180°C, the pellets deposited on the surface of solid residues were extracted gently with 1% sodium hydroxide (NaOH) solution. They were composed of 81.0% lignin, 7.0% glucan, and 3.2% xylan. The LHW pretreatment solution (PS) was sprayed to the filter paper, and the pellets were observed on its surface. Fourier transform infrared spectroscopy (FTIR) data showed that lignin was also the main component of the PS pellets. The effect of the pellets on enzymatic hydrolysis was chiefly attributed to the steric hindrance, not the cellulase adsorption. The structural characteristics of LHW-treated SCB might play a more important role in influencing the enzymatic hydrolysis than the pellets.
在液态热水（LHW）预处理过程中，木质纤维素表面会形成许多颗粒。本文研究了颗粒的特性及其对 LHW 处理甘蔗渣（SCB）酶解的影响。在 180°C 下用 LHW 处理 SCB 后，用 1%氢氧化钠（NaOH）溶液轻轻提取沉积在固体残渣表面的颗粒。它们由 81.0%木质素、7.0%葡聚糖和 3.2%木聚糖组成。将 LHW 预处理溶液（PS）喷洒到滤纸上，可以观察到其表面的颗粒。傅里叶变换红外光谱（FTIR）数据表明，PS 颗粒的主要成分也是木质素。颗粒对酶解的影响主要归因于空间位阻，而不是纤维素酶吸附。与颗粒相比，LHW 处理后的 SCB 的结构特征可能在影响酶解方面发挥更重要的作用。
