Zheng Zhenlong, Sohn Seonghyang, Ahn Keun Jae, Bang Dongsik, Cho Sung Bin
Department of Dermatology and Cutaneous Biology Research Institute, Yonsei University College of Medicine, Seoul, Korea.
Acta Derm Venereol. 2015 Nov;95(8):952-8. doi: 10.2340/00015555-2127.
Herpes simplex virus (HSV) infection is a possible pathogenic factor in Behçet's disease (BD). Using proteomics analysis, this study detected a target HSV protein. Serum IgA and IgG reactivities against the identified protein were evaluated in patients with BD and in BD-like mice. A total of 4 protein bands generated by immunoprecipitation were analysed by proteomics, and HSV UL48 was commonly found in both IgA- and IgG-reactive protein bands. Compared with controls, patients with BD and BD-like mice exhibited higher titres of IgA reacting with recombinant HSV UL48 protein. Further proteomics analysis revealed that human heat shock cognate 71 kDa protein (Hsc71) is a cross-reacting target antigen against anti-HSV UL48 antibody. In addition, our data demonstrated a very strong association between serum IgG reactivity against recombinant human Hsc71 and recombinant HSV UL48 in patients with BD. We suggest that HSV infection and impaired human Hsc71 activity may be associated with the activation of autoreactive lymphocytes.
单纯疱疹病毒（HSV）感染可能是白塞病（BD）的致病因素之一。本研究通过蛋白质组学分析检测到一种目标HSV蛋白。在BD患者和类BD小鼠中评估了血清中针对该已鉴定蛋白的IgA和IgG反应性。通过蛋白质组学分析免疫沉淀产生的总共4条蛋白条带，发现HSV UL48常见于IgA和IgG反应性蛋白条带中。与对照组相比，BD患者和类BD小鼠与重组HSV UL48蛋白反应的IgA滴度更高。进一步的蛋白质组学分析表明，人热休克同源71 kDa蛋白（Hsc71）是抗HSV UL48抗体的交叉反应靶抗原。此外，我们的数据表明，BD患者血清中针对重组人Hsc71和重组HSV UL48的IgG反应性之间存在非常强的关联。我们认为，HSV感染和人Hsc71活性受损可能与自身反应性淋巴细胞的激活有关。
