[xTAG GPP多重聚合酶链反应在感染性腹泻诊断中的应用及流行病学研究]

[The application and epidemiological research of xTAG GPP multiplex PCR in the diagnosis of infectious diarrhea].

作者信息

Feng Weiming, Gu Xiuli, Sui Wenjun, Zhang Mingxin, Lu Binghuai, Wang Mei, Huang Yanfei, Lu Xinxin

机构信息

Department of Laboratory Medicine, Capital Medical University, Beijing Tongren Hospital, Beijing 100710, China.

Department of Laboratory Medicine, Capital Medical University, Beijing Tongren Hospital, Beijing 100710, China. Email:

出版信息

Zhonghua Yi Xue Za Zhi. 2015 Feb 10;95(6):435-9.

PMID:25916780

Abstract

OBJECTIVE

To investigate the application value of xTAG (®) gastrointestinal pathogen panel (xTAG9(®) GPP) multiplex PCR in the early diagnosis of infectious diarrhea, and understand the epidemiology of intestinal diarrhea pathogens.

METHODS

Five hundred and ninety two specimens were collected in outpatient of Tongren Hospital, Capital Medical University, from 1st Oct 2013 to 30th Sep 2014, comparing the xTAG(®) GPP multiplex PCR assay with the traditional methods (culture, rapid enzyme immunoassay chromatography, microscopic examination, Real-time PCR) and mading the statistical analysis.

RESULTS

The positive rate of 592 patients with diarrhea specimens was 47.8% (283/592), the proportion of male and female was 1: 1.02, the average age was 31years. The virus detection rate was 18.1%, Rotavirus A was the most common organism detected (8.8%), concentrated in winter, popular in children.Secondly,Norovirus GI/GII (8.4%),Adenovirus 40/41 was five cases. The positive rate of bacteria was 35.5%, Enterotoxigenic E.coli (8.4%, 50/592) was most frequently detected in summer, common in young adults. The other pathogens were Campylobacter 7.7%, Salmonella 7.0%, Clostridium difficile toxinA/B 3.5%, Shigella 3.3%,E.coli O157 3.3% and Shiga toxin-producing E.coli LT/ST 1.7%.None of Yersinia enterocolitica and Vibrio cholerae was detected. There were ten samples with parasitic (1.7%), five samples were positive for Cryptosporidium, three for Entamoeba histolytica and two for Giardia. All of them did not have obvious distribution followed by season and population. Totally 242(40.8%) infected specimens with single pathogen were detected. There were 41 (6.9%) co-infections samples, including two pathogens 36 cases (6.1%), three pathogens in 5 cases (0.8%).

CONCLUSIONS

xTAG(®) GPP multiplex PCR is simple, sensitive, specific and can be used as a quick way to diagnose the infectious diarrhea. Diarrhea pathogen has significant characteristics with the season and crowd.

摘要

目的

探讨xTAG（®）胃肠道病原体检测板（xTAG9（®）GPP）多重PCR技术在感染性腹泻早期诊断中的应用价值，了解肠道腹泻病原体的流行病学特征。

方法

收集首都医科大学附属同仁医院2013年10月1日至2014年9月30日门诊腹泻患者标本592份，将xTAG（®）GPP多重PCR检测方法与传统检测方法（培养法、快速酶免疫层析法、显微镜检查、实时荧光定量PCR）进行比较，并做统计学分析。

结果

592例腹泻患者标本阳性率为47.8%（283/592），男女比例为1∶1.02，平均年龄31岁。病毒检出率为18.1%，其中A组轮状病毒检出最多（8.8%），集中在冬季，以儿童为主；其次为诺如病毒GI/GII（8.4%），腺病毒40/41共5例。细菌阳性率为35.5%，产肠毒素大肠埃希菌（8.4%，50/592）检出最多，集中在夏季，以青壮年为主。其他病原体有空肠弯曲菌7.7%、沙门菌7.0%、艰难梭菌毒素A/B 3.5%、志贺菌3.3%、大肠埃希菌O157 3.3%、产志贺毒素大肠埃希菌LT/ST 1.7%。未检出小肠结肠炎耶尔森菌和霍乱弧菌。寄生虫阳性标本10份（1.7%），其中隐孢子虫5份、溶组织内阿米巴3份、蓝氏贾第鞭毛虫2份，均无明显的季节及人群分布特点。共检出单一病原体感染标本242份（40.8%），混合感染标本41份（6.9%），其中两种病原体感染36例（6.1%），三种病原体感染5例（0.8%）。