Fornés M W, de Rosas J C
Instituto de Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina.
Gamete Res. 1989 Dec;24(4):453-9. doi: 10.1002/mrd.1120240411.
Morphological changes in sperm are one aspect of a maturation process during epididymal transit in mammals. The literature mentions only, for different strains of rats, a remodeling and decrease in size of the acrosome. In the present work, the sperm were obtained from caput, corpus, and cauda epididymis of the albino rat. Samples were processed for scanning electron microscopy, with routine techniques, and for light microscopy and video microscopy. It appeared, with these techniques, that the acrosomal curvature and the whole head surface area of the rat sperm decrease during the epididymal transit. To measure these changes, a geometrical method was designed, and surface measurements were made using a computer program. It was found that the caput sperm head has the greatest surface area and a sharper acrosome bend than the cauda sperm. In an attempt to explain the above-mentioned changes, the suggestion is offered that some compactation of the nucleus and acrosomal material could be related to the decrease of the surface area.
精子的形态变化是哺乳动物附睾转运过程中成熟过程的一个方面。文献仅提及不同品系大鼠的顶体有重塑和尺寸减小的情况。在本研究中，精子取自白化大鼠附睾的头部、体部和尾部。样本采用常规技术进行扫描电子显微镜处理，以及光学显微镜和视频显微镜处理。通过这些技术发现，大鼠精子的顶体曲率和整个头部表面积在附睾转运过程中会减小。为了测量这些变化，设计了一种几何方法，并使用计算机程序进行表面测量。结果发现，附睾头部精子的头部表面积最大，顶体弯曲比附睾尾部精子更明显。为了解释上述变化，有人提出细胞核和顶体物质的一些致密化可能与表面积的减小有关。
