含肽片段的新型两亲性对叔丁基硫杂杯[4]芳烃的合成及其与DNA的相互作用。

The synthesis of new amphiphilic p-tert-butylthiacalix[4]arenes containing peptide fragments and their interaction with DNA.

作者信息

Padnya Pavel L, Andreyko Elena A, Mostovaya Olga A, Rizvanov Ildar Kh, Stoikov Ivan I

机构信息

Kazan (Volga Region) Federal University, A.M. Butlerov Chemical Institute, 420008 Kremlevskaya, 18, Kazan, Russian Federation.

出版信息

Org Biomol Chem. 2015 Jun 7;13(21):5894-904. doi: 10.1039/c5ob00548e.

DOI:10.1039/c5ob00548e

PMID:25921225

Abstract

New water-soluble p-tert-butylthiacalix[4]arenes containing peptide and quaternary ammonium fragments in cone and 1,3-alternate conformations were synthesized and characterized. The interaction of the macrocycles with DNA was studied by UV-spectroscopy, DLS and TEM. It was shown that the interaction of the self-associates based on p-tert-butylthiacalix[4]arenes tetrasubstituted at the lower rim with glycine and quaternary ammonium fragments in cone and 1,3-alternate conformations with DNA led to the formation of particles of about 99-192 nm in size.

摘要

合成并表征了在锥形和1,3-交替构象中含有肽和季铵片段的新型水溶性对叔丁基硫杂杯[4]芳烃。通过紫外光谱、动态光散射和透射电子显微镜研究了大环与DNA的相互作用。结果表明，基于在下缘四取代的对叔丁基硫杂杯[4]芳烃与甘氨酸和季铵片段以锥形和1,3-交替构象形成的自缔合体与DNA的相互作用导致形成尺寸约为99-192nm的颗粒。