Stenzel D J, Dunn L A, Boreham P F
Int J Parasitol. 1989 Nov;19(7):787-91. doi: 10.1016/0020-7519(89)90067-2.
A study of the function of the electron-dense pits in the vacuolar and granular forms of Blastocystis hominis was undertaken. Immuno-electron microscopy using anti-clathrin antibody and colloidal gold demonstrated clathrin to be associated with all forms of the pits and some cytoplasmic vesicles. Cationized ferritin traced the pathway of endocytosis from the surface of the coated pits through internalization via electron-dense coated vesicles and uncoated vesicles and tubules in the cytoplasm. The cationized ferritin particles accumulated in the central vacuole, suggesting a metabolic or storage role for this structure. Differences in the accumulation of cationized ferritin particles were noted between vacuolar and granular forms. The hydrolytic enzyme acid phosphatase was not detected within the central vacuole suggesting that this structure does not act as a lysosome.
对人芽囊原虫空泡型和颗粒型中电子致密小窝的功能进行了研究。使用抗网格蛋白抗体和胶体金的免疫电子显微镜显示，网格蛋白与所有形式的小窝和一些细胞质囊泡相关。阳离子铁蛋白追踪了从被膜小窝表面通过内化，经电子致密被膜囊泡、无被膜囊泡和细胞质中的小管的内吞途径。阳离子铁蛋白颗粒积聚在中央液泡中，表明该结构具有代谢或储存作用。在空泡型和颗粒型之间观察到阳离子铁蛋白颗粒积累的差异。在中央液泡内未检测到水解酶酸性磷酸酶，表明该结构不起溶酶体的作用。
