Dubrovsky Anna, Sorrentino Simona, Harapin Jan, Sapra K Tanuj, Medalia Ohad
Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.
Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland; Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben-Gurion University, Beer-Sheva 84105, Israel.
Arch Biochem Biophys. 2015 Sep 1;581:78-85. doi: 10.1016/j.abb.2015.04.006. Epub 2015 Apr 25.
Structural analysis of macromolecular assemblies and their remodeling during physiological processes is instrumental to defining the fundament of cellular and molecular biology. Recent advances in computational and analytical tools for cryo-electron tomography have enabled the study of macromolecular structures in their native environment, providing unprecedented insights into cell function. Moreover, the recent implementation of direct electron detectors has progressed cryo-electron tomography to a stage where it can now be applied to the reconstruction of macromolecular structures at high resolutions. Here, we discuss some of the recent technical developments in cryo-electron tomography to reveal structures of macromolecular complexes in their physiological medium, focusing mainly on eukaryotic cells.
对大分子组装体及其在生理过程中的重塑进行结构分析，有助于确定细胞和分子生物学的基础。用于冷冻电子断层扫描的计算和分析工具的最新进展，使得在天然环境中研究大分子结构成为可能，为细胞功能提供了前所未有的见解。此外，直接电子探测器的最新应用已将冷冻电子断层扫描推进到一个新阶段，现在它可用于高分辨率重建大分子结构。在此，我们讨论冷冻电子断层扫描的一些最新技术发展，以揭示生理介质中大分子复合物的结构，主要聚焦于真核细胞。
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