[雌激素诱导的细胞内钙快速增加在小鼠囊胚着床中起重要作用]

[Estrogen induced rapid increase of intracellular calcium plays important role in the implantation of mouse blastocysts].

作者信息

Zhang Shi-mao, Yu Lin-lin, Qu Ting, Yuan Dong-zhi, Zhang Jin-hu, He Ya-ping, Yue Li-min

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2015 Mar;46(2):205-8.

Abstract

OBJECTIVE

To study the roles of the increased intracellular calcium induced rapidly by estrogen in the implantation of mouse blastocysts.

METHODS

The mouse blastocysts were collected from the female mice on the pregnant day 4, divided into 3 groups: control, E2-BSA and BAPTA +E2-BSA. Immunofluorescence staining, confocal microscopy, embryo and endometrial epithenial cells co-culture and embryo transfer were used to investigate the effect of increased intracellular calcium induced by E2-BSA on the expression and localization of integrins in blastocysts and their adhesion to endometrial epithenial calls (EECs) and implantation into the endometrium.

RESULTS

The increase of intracellular calcium induced rapidly by estrogen could cause the cluster and relocation of integrin av and beta3, and BAPTA might block this effect, the adhesion rate of blastocysts in contol group was 35.5%, BAPTA +E2-BSA group was 26.7% and significantly lower than 65.6% of E2-BSA group (P<0.05), and the implantation rate in BAPTA+E2-BSA group was 11.8%, which was significantly lower than 52.9% of E2-BSA group (P<0.05).

CONCLUSION

The rapid increase of intracellular calcium induced by estrogen may cause the relocalization of integrin in blastocysts and their adhesion to ECCs, which is important in the process of implantation.

摘要

目的

研究雌激素快速诱导的细胞内钙升高在小鼠胚泡着床中的作用。

方法

于妊娠第4天从雌性小鼠收集胚泡，分为3组：对照组、E2-BSA组和BAPTA+E2-BSA组。采用免疫荧光染色、共聚焦显微镜、胚胎与子宫内膜上皮细胞共培养及胚胎移植等方法，研究E2-BSA诱导的细胞内钙升高对胚泡中整合素表达和定位及其与子宫内膜上皮细胞（EECs）黏附及着床于子宫内膜的影响。

结果

雌激素快速诱导的细胞内钙升高可导致整合素αv和β3聚集和重新定位，BAPTA可阻断此效应，对照组胚泡黏附率为35.5%，BAPTA+E2-BSA组为26.7%，显著低于E2-BSA组的65.6%（P<0.05），BAPTA+E2-BSA组着床率为11.8%，显著低于E2-BSA组的52.9%（P<0.05）。