Cui Hong-xia, Wang Ming, Yuan Jia-xin, Liu Ji-cheng
Yao Xue Xue Bao. 2015 Jan;50(1):45-9.
This study is to investigate the effect of ethyl gallate on invasion capabilities and its mechanism of breast cancer MDA-MB-231 cells. Using cell adhesion and transwell assay, separately, the effects of ethyl gallate on the invasion of MDA-MB-231 cells were measured. The Akt-NF-κB signal pathway protein expressions were analyzed with Western blot. Also, the mRNA levels of MMP-9 and MMP-2 were analyzed by RT-PCR. Ethyl gallate inhibited the abilities of motility, adhesion and invasion of breast cancer MDA-MB-231 cells in vitro (P<0.05), inhibited the mRNA levels of MMP-9, MMP-2, phosphorylation of AKt and protein expression of NF-κB. It is concluded that ethyl gallate can inhibit the abilities of invasion of breast cancer in vitro by inhibiting the mRNA levels of MMP-9/MMP-2, phosphorylation of Akt and protein expression of NF-κB.
本研究旨在探讨没食子酸乙酯对乳腺癌MDA-MB-231细胞侵袭能力的影响及其作用机制。分别采用细胞黏附实验和Transwell实验检测没食子酸乙酯对MDA-MB-231细胞侵袭的影响。采用蛋白质免疫印迹法分析Akt-NF-κB信号通路蛋白表达。同时，通过逆转录聚合酶链反应分析基质金属蛋白酶-9（MMP-9）和基质金属蛋白酶-2（MMP-2）的mRNA水平。没食子酸乙酯在体外可抑制乳腺癌MDA-MB-231细胞的运动、黏附及侵袭能力（P<0.05），抑制MMP-9、MMP-2的mRNA水平、Akt的磷酸化及NF-κB的蛋白表达。结论：没食子酸乙酯可通过抑制MMP-9/MMP-2的mRNA水平、Akt的磷酸化及NF-κB的蛋白表达，在体外抑制乳腺癌的侵袭能力。
