Moridian M, Khorsandi L, Talebi A R
Bratisl Lek Listy. 2015;116(5):321-5. doi: 10.4149/bll_2015_060.
To evaluate the effects of zinc oxide nanoparticles (ZNPs) on morphometric and stereological parameters of mouse testis.
ZNPs are increasingly used in sunscreens, biosensors, food additives, pigments, rubber manufacture, and electronic materials. However, the potential toxicity of these nanoparticles is not well understood.
Experimental Groups (ZNP-1, ZNP-2 and ZNP-3) received one of the following treatments daily for 35 days: 5, 50 and 300 mg/kg ZNPs respectively. Right testis from each animal was fixed in bouin's solution for measurement of total volume of testis, total volume of seminiferous tubules, total volume of interstitial tissue and total number of Leydig cells by stereological methods. Seminiferous tubule diameter and seminiferous epithelium height were assessed by morphometrical method. The left testicles were homogenized for measurement of testosterone concentration.
There was a significant decrease in the weight of testicles in ZNP-3 groups. The stereological and morphometrical parameters were significantly changed in ZNP-2 and ZNP-3 groups. ZNP-2 and ZNP-3 groups also showed a significant decrease in testosterone concentrations (p < 0.05).
This study demonstrated that ZNPs can change stereological and morphometrical parameters of the seminiferous tubules and reduce the number of Leydig cells (Tab. 3, Fig. 3, Ref. 36).
评估氧化锌纳米颗粒（ZNPs）对小鼠睾丸形态测量和体视学参数的影响。
ZNPs越来越多地用于防晒霜、生物传感器、食品添加剂、颜料、橡胶制造和电子材料中。然而，这些纳米颗粒的潜在毒性尚未得到充分了解。
实验组（ZNP - 1、ZNP - 2和ZNP - 3）每天接受以下治疗之一，持续35天：分别为5、50和300mg/kg ZNPs。通过体视学方法，将每只动物的右侧睾丸固定在布因氏溶液中，用于测量睾丸总体积、生精小管总体积、间质组织总体积和Leydig细胞总数。通过形态测量法评估生精小管直径和生精上皮高度。将左侧睾丸匀浆以测量睾酮浓度。
ZNP - 3组睾丸重量显著下降。ZNP - 2和ZNP - 3组的体视学和形态测量参数发生了显著变化。ZNP - 2和ZNP - 3组的睾酮浓度也显著降低（p < 0.05）。
本研究表明，ZNPs可改变生精小管的体视学和形态测量参数，并减少Leydig细胞数量（表3，图3，参考文献36）。
