Demir N, Nadaroglu H, Gungor A A, Demir Y
Bratisl Lek Listy. 2015;116(5):330-3. doi: 10.4149/bll_2015_062.
The influence of prostatic acid phosphatase (PAP) and human chorionic gonadotropin (HCG), tumor markers have been investigated on human erythrocyte carbonic anhydrase (HCA-I and HCA-II) and bovine erythrocyte (BCA) and bovine lung carbonic anhydrase (CA-IV) in vitro.
Tumor markers are substances that can often be detected in higher-than-normal amounts in the blood, urine, or body tissues of some patients with certain types of cancer. Tumor markers are produced either by the tumor itself or by the body in response to the presence of cancer or certain benign (noncancerous) conditions. In addition to their role in cancer diagnosis, some tumor marker levels are measured before treatment to help doctors plan appropriate therapy.
All of the tumor markers were determined to have inhibition effect, on human CA-I, CA-II, bovine erythrocyte CA (BCA) and bovine lung CA-IV isoenzymes. The effect of each tumor marker on CA was investigated by Wilbur-Andersen method modified by Rickly et al Inhibition effects of two different tumor markers on human CA-I, CA-II, bovine erythrocyte CA (BCA) and bovine lung CA-IV isoenzymes were determined by using the CO2-Hydratase method by plotting activity % vs (tumor markers). I50 values of tumor markers exhibiting inhibition effects were found by means of these graphs (Tab.1, Fig. 2, Ref. 20).
体外研究前列腺酸性磷酸酶（PAP）和人绒毛膜促性腺激素（HCG）这两种肿瘤标志物对人红细胞碳酸酐酶（HCA-I和HCA-II）、牛红细胞碳酸酐酶（BCA）以及牛肺碳酸酐酶（CA-IV）的影响。
肿瘤标志物是在某些患有特定类型癌症的患者的血液、尿液或身体组织中，常常能够检测到含量高于正常水平的物质。肿瘤标志物由肿瘤本身产生，或者由机体在对癌症或某些良性（非癌性）病症作出反应时产生。除了在癌症诊断中的作用外，在治疗前测量一些肿瘤标志物水平有助于医生制定合适的治疗方案。
所有肿瘤标志物均被确定对人CA-I、CA-II、牛红细胞CA（BCA）和牛肺CA-IV同工酶具有抑制作用。通过Rickly等人改良的Wilbur-Andersen方法研究了每种肿瘤标志物对CA的影响。通过CO2-水合酶方法，绘制活性百分比对（肿瘤标志物）的曲线，测定了两种不同肿瘤标志物对人CA-I、CA-II、牛红细胞CA（BCA）和牛肺CA-IV同工酶的抑制作用。借助这些图表（表1，图2，参考文献20）找到了表现出抑制作用的肿瘤标志物的I50值。
