Capillary zone electrophoresis of peptide fragments from trypsin digestion of biosynthetic human growth hormone.

Capillary zone electrophoresis (CZE) was applied to the separation of the 19 peptide fragments produced by enzymatic digestion of human growth hormone (hGH). The fragments of hGH produced by trypsin digestion under non-reducing conditions were identified in the electropherogram. Almost all of the fragments were resolved by CZE in less than 15 min. There is a marked difference in selectivity between reversed-phase high-performance liquid chromatography (RP-HPLC) and CZE. CZE is demonstrated to be a powerful complement to RP-HPLC for routine identification of hGH using trypsin digests.