Nielsen R G, Riggin R M, Rickard E C
Eli Lilly and Company, Department MC 772, Indianapolis, IN 46285.
J Chromatogr. 1989 Oct 20;480:393-401. doi: 10.1016/s0021-9673(01)84308-9.
Capillary zone electrophoresis (CZE) was applied to the separation of the 19 peptide fragments produced by enzymatic digestion of human growth hormone (hGH). The fragments of hGH produced by trypsin digestion under non-reducing conditions were identified in the electropherogram. Almost all of the fragments were resolved by CZE in less than 15 min. There is a marked difference in selectivity between reversed-phase high-performance liquid chromatography (RP-HPLC) and CZE. CZE is demonstrated to be a powerful complement to RP-HPLC for routine identification of hGH using trypsin digests.
毛细管区带电泳（CZE）用于分离人生长激素（hGH）酶解产生的19个肽片段。在非还原条件下胰蛋白酶消化产生的hGH片段在电泳图中得到了鉴定。几乎所有片段在15分钟内通过CZE得到分离。反相高效液相色谱（RP-HPLC）和CZE在选择性上存在显著差异。结果表明，CZE是利用胰蛋白酶消化产物对hGH进行常规鉴定的一种强大的RP-HPLC补充方法。
